ABCB5 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-93365
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Summary for ABCB5 Antibody
Immunogen
Peptide with sequence C-QTQHRNTSKKAQ corresponding to internal region according to NP_848654.3, NP_001157413.1.
Epitope
C-QTQHRNTSKKAQ
Specificity
This antibody is expected to recognise both reported isoforms (NP_848654.3; NP_001157413.1)
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Theoretical MW
90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ABCB5 Antibody
Western Blot: ABCB5 Antibody [NB100-93365] - (0.3ug/ml) staining of lysates of cell line A431 (35ug protein in RIPA buffer). Detected by chemiluminescence.
Flow Cytometry: ABCB5 Antibody [NB100-93365] - analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Applications for ABCB5 Antibody
Application
Recommended Usage
Flow Cytometry
10 ug
Peptide ELISA
Detection limit 1:32000
Western Blot
0.3 - 1 ug/ml
Application Notes
FLOW successful usage on Hep3B cells is reported in scientific literature (PMID: 20682318) and tested in HepG2 Cells
WB: Approx. 80 kDa band observed in lysates of A431(calculated MW of 89.8 kDa band according to NP_848654.3). Primary incubation was 1 hour.
WB: Approx. 80 kDa band observed in lysates of A431(calculated MW of 89.8 kDa band according to NP_848654.3). Primary incubation was 1 hour.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ABCB5
Long Name
ATP-binding Cassette, Sub-family B (MDR/TAP), Member 5
Alternate Names
ABCB5 P-gp, ABCB5alpha, ABCB5beta, ATP-binding cassette protein, ATP-binding cassette sub-family B member 5, ATP-binding cassette, sub-family B (MDR/TAP), member 5, EC 3.6.3, EC 3.6.3.44, EST422562, P-glycoprotein ABCB5
Entrez Gene IDs
340273 (Human)
Gene Symbol
ABCB5
UniProt
Additional ABCB5 Products
Product Documents for ABCB5 Antibody
Product Specific Notices for ABCB5 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...