Simple Western Antibody Database

Find Antibodies Validated for Simple Western

Simple Western™ systems are open platforms, meaning the possibilities are almost endless when selecting antibodies to develop your assays. The Simple Western Antibody database is curated to facilitate the selection process by providing general assay development guidance for identifying and selecting antibodies to test. These guidelines are provided to save time with recommended starting points to optimize for the biological system being examined.

How do antibodies become validated for Simple Western?

R&D Systems, Novus Biologicals, and valued partners such as Cell Signaling Technology and Bethyl Laboratories are continually validating their antibodies for use with Simple Western.
Help other scientists and fund your research! Submit new antibody validation data to the Simple Western antibody database and receive a free 8x25 separation module or detection module. Learn more about the Simple Western Antibody Validation Promotion.
Our expert in-house scientists continue to validate antibodies and review customer-submitted data to ensure the recommendations meet our high standards, so come back for the latest list of validated antibodies in the future.

You can sort by column headings or just type into the Search box. If you don't see your antibody listed here, email us at support@proteinsimple.com.

*Antibodies validated and backed by Cell Signaling Technology (CST)

Simple Western Validated Antibodies

Filter Antibodies: (5420)

Protein Target	Antibody Type	Vendor / Cat #	Protein Isoform	Citations & Supporting Data	Host Species	Sample	Separation	Dilution	kDa on Simple Western
α 1-Acid Glycoprotein	Primary	R&D Systems AF3694		Datasheet	Goat	Human serum	Size	5 µg/mL	79
α 1-Microglobulin	Primary	R&D Systems MAB7720		Datasheet	Mouse	Rat plasma	Size	20 µg/mL	37
α 1B-Glycoprotein	Primary	R&D Systems AF7757		Datasheet	Sheep	Human liver tissue	Size	10 µg/mL	94
α 1B-Glycoprotein	Primary	R&D Systems AF8537		Datasheet	Rabbit	Mouse plasma and rat plasma	Size	1:100	113
α 2-Macroglobulin	Primary	R&D Systems AF1938		Datasheet	Goat	Human serum	Size	1 µg/mL	178
α 2-Macroglobulin	Primary	R&D Systems MAB1938		Datasheet	Mouse	Human serum	Size	10 µg/mL	178
α fodrin	Primary	Enzo Life Sciences BML-FG6090			Mouse	Cell lysate, BAL fluid, plasma, serum	Size	1:10, 1:50	150, 250
α Synuclein	Primary	Novus Biologicals NBP1-05194		Datasheet	Mouse	Human Brain lysate 0.05 mg/mL	Size	1:2000	20
α Synuclein	Primary	BD Biosciences 610786			Mouse	Mouse striatum, mouse adrenal, rat adrenal, rat striatum	Size	1:25	21
α tubulin	Primary	Novus Biologicals NB100-690		Citations (5) Datasheet	Mouse	HeLa lysate 1.0 mg/mL, Titrated to saturation using various models	Size	1:50, 6 µg/mL	55
α Tubulin	Primary	Abcam ab4074		Citations (1) Datasheet	Rabbit		Size	1:1000	53
α Tubulin 4a	Primary	Novus Biologicals NB120-11304		Datasheet	Mouse	Caco2 cell lysates	Size	1:50
α-actinin	Primary	Santa Cruz Biotechnology sc-17829		Citations (1) Datasheet	Mouse	NCI-H1975	Size
α-Cardiac Actin	Primary	R&D Systems MAB9308		Datasheet	Mouse	Human, mouse, and rat heart tissue	Size	2 µg/mL	50
α-Cardiac Actin	Primary	R&D Systems MAB93081		Datasheet	Mouse	Human Heart	Size	1:20	49
α-Fetoprotein/AFP	Primary	R&D Systems MAB13692		Datasheet	Mouse	HepG2 and HepB3 lysate 0.2 mg/mL	Size	5 µg/mL	67-68
α-Fetoprotein/AFP	Primary	R&D Systems AF1369		Datasheet	Chicken	HepG2 human hepatocellular carcinoma cell line	Size	2 µg/mL	70
α-Fetoprotein/AFP	Primary	R&D Systems MAB1368		Datasheet	Mouse	HepG2 human hepatocellular carcinoma cell line	Size	10 µg/mL	70
α-Fetoprotein/AFP	Primary	Novus Biologicals NB110-7961		Datasheet	Mouse		Size
α-Galactosidase A/GLA	Primary	R&D Systems AF6146		Datasheet	Sheep	MCF-7 human breast cancer cell line and HeLa human cervical epithelial carcinoma cell line 0.2 mg/mL	Size	50 µg/mL	55
α-Internexin	Primary	Novus Biologicals NB300-140		Datasheet	Mouse	SH-SY5Y lysate	Size	1:200	140
α-Nucleocapsid	Primary	Novus Biologicals NB100-56683	SARS Nucleocapsid Protein	Datasheet	Rabbit	Recombinant SARS-CoV-2 Nucleocapsid Protein (Novus Biologicals PN 10474-CV); Lysates of SARS-CoV-2)	Size	25 mg/mL	60
α-Smooth Muscle Actin	Primary	R&D Systems MAB1420		Datasheet	Mouse	iBJ6 human induced pluripotent stem cell line differentiated to cardiomyocytes	Size	10 µg/mL	49
α-Smooth Muscle Actin (D4K9N)*	Primary	Cell Signaling Technology 19245	Human Specific	Citations (2) Datasheet	Rabbit	Human Colon	Size	1:10–1:50	48, 49
α-Synuclein	Primary	R&D Systems AF1338		Datasheet	Goat	Sk-Mel-28	Size	1:10	21
α-Synuclein	Primary	Cell Signaling Technology 2642			Rabbit		Size	1:25	21
α-synuclein	Primary	Proteintech 10842-1-AP	Unmodified	Citations (1) Datasheet	Rabbit	Heart	Size	1:100
α-Synuclein (E4U2F)*	Primary	Cell Signaling Technology 51510		Datasheet	Rabbit	COS-7 + HA-Stat3	Size	1:10–1:50	20
α-Synuclein (Syn204)	Primary	Cell Signaling Technology 2647		Citations (1) Datasheet	Mouse	Spinal cord 3 µg/µL	Size	1:10	22
α-Synuclein, Phospho	Primary	Cell Signaling Technology 93706	Phospho-Ser129		Rabbit	Spinal cord 3 µg/µL	Size	1:250	23
α-Synuclein, Phospho	Primary	Cell Signaling Technology 23706	Phospho-Ser129		Rabbit	Spinal cord 3 µg/µL	Size	1:250	23
α-Tubulin (11H10)*	Primary	Cell Signaling Technology 2125		Citations (5) Datasheet	Rabbit	COS-7	Size	1:10–1:50	55
α-Tubulin (DM1A)*	Primary	Cell Signaling Technology 3873		Citations (2) Datasheet	Mouse	SupM2	Size	1:10–1:50	55
αA Crystallin/CRYAA	Primary	R&D Systems AF4848		Datasheet	Goat	Mouse eye lens tissue	Size	1 µg/mL	28
αB Crystallin/CRYAB	Primary	R&D Systems AF4849		Datasheet	Goat	Human heart tissue and mouse heart tissue	Size	2 µg/mL	29
αB Crystallin/CRYAB	Primary	R&D Systems MAB4849		Datasheet	Mouse	Human heart tissue	Size	1 µg/mL	28
αB Crystallin/CRYAB	Primary	Proteintech 68001-1-Ig		Datasheet	Mouse	HSMM – Human Skeletal Muscle Myoblasts whole-cell lysate	Size	1:10, 1:50, 1:250	28
β 2 Microglobulin	Primary	Abcam ab75853	Unmodified	Citations (2) Datasheet	Rabbit	Many	Charge	1:50
β actin	Primary	Novus Biologicals NB100-56874		Datasheet	Rabbit	HeLa lysate 1.0 mg/mL	Size	1:12.5	49
β actin	Primary	Novus Biologicals NB600-503		Citations (1) Datasheet	Rabbit	HeLa lysate 0.1 mg/mL	Size	1:12.5	50
β actin	Primary	Novus Biologicals NBP1-47423		Citations (1) Datasheet	Mouse	HeLa lysate 1.0 mg/mL	Size	12.5	49
β Actin	Primary	Abcam ab6276		Citations (8) Datasheet	Mouse	Blood	Size	1:25–1:100
β Actin	Primary	Abcam ab8224		Citations (3) Datasheet	Mouse	Blood	Size	1:100	49
β Actin	Primary	Abcam ab8226		Citations (2) Datasheet	Mouse	HeLa, Jurkat, HEK 293	Size	1:50	48
β actin	Primary	Proteintech HRP-60008			Mouse	HeLa cell lysates	Size	1:50	47
β Adducin	Primary	Novus Biologicals NBP2-33974		Datasheet	Rabbit		Size
β amyloid	Primary	Cell Signaling Technology 2454			Rabbit	HeLa lysate spiked with b-amyloid	Size	1:100	3
β amyloid	Primary	BioLegend 803015	β amyloid amino acid position 1-16		Mouse	Cerebro Spibal Fluid (CSF) peptides	Charge	1:500
β arrestin	Primary	Novus Biologicals NBP2-24569		Datasheet	Rabbit	Jurkat lysate 0.5 mg/mL	Size	1:100	54
β catenin	Primary	Novus Biologicals NBP1-54467		Citations (1) Datasheet	Mouse	HepG2 lysate 0.5 mg/mL	Size	1:100	90

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Simple Western Antibody Database

Find Antibodies Validated for Simple Western

Simple Western Validated Antibodies

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