Rat alpha1-Microglobulin Antibody

Name: Rat alpha1-Microglobulin Antibody
Brand: R&D Systems
SKU: MAB7720

R&D Systems, part of Bio-Techne | Catalog # MAB7720

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Rat

Applications

Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 771803

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Product Summary for Rat alpha1-Microglobulin Antibody

Immunogen

E. coli-derived recombinant rat alpha1‑Microglobulin
Asp20-Ala202
Accession # Q64240

Specificity

Detects rat alpha1‑Microglobulin in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human alpha1-Microglobulin is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Rat alpha1-Microglobulin Antibody

Detection of Rat alpha1‑Microglobulin by Western Blot.

Western blot shows rat plasma. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Rat a1-Microglobulin Monoclonal Antibody (Catalog # MAB7720) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for a1-Microglobulin at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Rat alpha1‑Microglobulin by Simple Western^TM.

Simple Western lane view shows rat plasma, loaded at 0.5 mg/mL. A specific band was detected for alpha1‑Microglobulin at approximately 37 kDa (as indicated) using 20 µg/mL of Mouse Anti-Rat alpha1‑Microglobulin Monoclonal Antibody (Catalog # MAB7720). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Rat alpha1-Microglobulin Antibody

Application

Recommended Usage

Simple Western

20 µg/mL
Sample: Rat plasma

Western Blot

2 µg/mL
Sample: Rat plasma

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha 1-Microglobulin

Rat alpha1-Microglobulin (alpha1-m/A1M; also protein HC) is a secreted 25-26 kDa glycoprotein member of the lipocalin family, calycin superfamily of molecules. It is expressed by hepatocytes, keratinocytes, and endodermal derivatives in the embryo. alpha1‑Microglobulin appears to act as a heme scavenger, protecting cells and collagen against oxidative damage. It also acts as an immunosuppressant, inhibiting polyclonal lymphocyte activation and dampening granulocyte migration in response to chemokines. alpha1‑Microglobulin circulates either as a monomer, or bound to IgA, albumin or prothrombin. Rat alpha1‑Microglobulin is generated through cleavage of a precursor molecule termed AMBP. This AMBP should not be confused with AMBP-1, a 120-140 kDa adrenomedullin-binding protein that is also known as Complement Factor H. The AMBP precursor contains a 19 aa signal sequence, an N-terminal 183 aa alpha1‑Microglobulin protein (aa 20-202), and a C-terminal serine protease inhibitor termed bikunin (aa 205-349). alpha1‑Microglobulin possesses one lipocalin domain (aa 41-186). Although cleavage of AMBP in the Golgi apparatus typically generates a 25 kDa alpha1‑Microglobulin and 28 kDa bikunin molecule, the 55-65 kDa AMBP precursor can also be released intact. In human, alpha1‑Microglobulin will undergo extracellular processing, generating an isoform that is missing the C-terminal four amino acids. There are four potential isoform variants. One utilizes an alternative start site at Met181, a second contains an Asn substitution for aa 112-228, a third possesses an 11 aa substitution for aa 1-141, and a fourth shows a 17 aa substitution for aa 201-349. Over aa 20-202, rat alpha1‑Microglobulin shares 76% and 86% aa sequence identity with human and mouse alpha1‑Microglobulin, respectively

Alternate Names

A1M, alpha 1Microglobulin, AMBP, EDC1, HCP, HI30, IATIL, ITI, ITIL, ITILC, UTI

Entrez Gene IDs

259 (Human); 11699 (Mouse); 25377 (Rat)

Gene Symbol

AMBP

UniProt

Q64240

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Product Specific Notices for Rat alpha1-Microglobulin Antibody

For research use only

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