SARS Nucleocapsid Protein Antibody

Simple Western: SARS Nucleocapsid Protein Antibody [NB100-56683] - Simple Western lane view shows recombinant SARS-CoV-2 Nucleocapsid Protein (Catalog # 10474-CV), loaded at 20 ng/mL. A specific band was detected for SARS-CoV-2 Nucleocapsid Protein at approximately 60 kDa (as indicated) using a serial dilution of Rabbit Anti-SARS-CoV Nucleocapsid Protein Polyclonal Antibody (Catalog # NB100-56683) followed by incubation with HRP-conjugated Anti-Goat IgG Secondary Antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Dual RNAscope ISH-IHC: SARS Nucleocapsid Protein Antibody [NB100-56683] - Formalin-fixed paraffin-embedded tissue sections of SARS-CoV-2 infected human lung tissue were probed for SARS-CoV-2 viral RNA (ACD anti-sense specific probe v-nCoV2019-S [848561]); Fast Red chromogen, ACD [322360]). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal anti-SARS Nucleocapsid Antibody [NB100-56683] at 15ug/mL with 1 hr incubation at 25 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to SARS-CoV-2 infected cells.

Simple Western: SARS Nucleocapsid Protein Antibody [NB100-56683] - Simple Western lane view shows lysates of SARS-CoV-2 (1:50), MERS (1:100), OC43 (1:100), and 229E (1:100). A specific band was detected for SARS-CoV-2 Nucleocapsid Protein at approximately 60 kDa (as indicated) only in the SARS-CoV-2 lysate using 25 ug/mL of Rabbit Anti-SARS-CoV Nucleocapsid Protein Polyclonal Antibody (Catalog # NB100-56683) followed by incubation with HRP-conjugated Anti-Goat IgG Secondary Antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Note: some reactivity observed with FL Std 230. SARS-CoV-2 lysate courtesy of University of Maryland.