Actin Antibody (3B3) - BSA Free

Validated:

Unconjugated

Monoclonal Mouse IgG₁ Clone # 3B3

BSA Free

1 mg/ml

This antibody is a mouse monoclonal IgG1 antibody derived by fusion of NS0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a peptide comprising the N-terminal nonapeptide of alpha-skeletal actin with an acetylated N-terminus (Ac-DEDETTALVC-COOH) coupled to keyhole limpet hemocyanin through the cysteine residue.

The epitope recognized by Clone 3B3 is highly conserved and the antibody is therefore expected to react with many other species.

This antibody highly specific for alpha-skeletal actin, and does not cross react with other actin isoforms.

Monoclonal

Mouse

IgG₁

The antibody is shipped at ambient temperature and may be stored at 4C. For prolonged storage prepare appropriate aliquots and store at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Protein A or G purified

PBS, pH 7.2

BSA Free

0.09% Sodium Azide

1 mg/ml

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.