Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Cited:

FLOW, ICC/IF, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # ER-BMDM1

Format

BSA Free

Concentration

1.0 mg/ml

View all Aminopeptidase N/CD13 Antibodies »

Product Specifications

Immunogen

Aminopeptidase N/CD13 Antibody (ER-BMDM1) made from Balb/c bone marrow derived macrophages.

Localization

Cell membrane; single-pass type II membrane protein

Specificity

NB100-64843 recognizes the murine homologue of human CD13.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Aminopeptidase N/CD13 Antibody (ER-BMDM1) - BSA Free

Immunohistochemistry-Paraffin: Aminopeptidase N/CD13 Antibody (ER-BMDM1) [NB100-64843] - Analysis of an FFPE tissue section of the mouse lymph node using 1:200 dilution of CD13 antibody.
Flow Cytometry: Aminopeptidase N/CD13 Antibody (ER-BMDM1) [NB100-64843] - Staining of mouse peripheral blood granulocytes with rat anti-mouse CD13.
Immunohistochemistry-Frozen: Aminopeptidase N/CD13 Antibody (ER-BMDM1) [NB100-64843] - Mouse lymph node cryosection anti-CD13 antibody.
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Applications for Aminopeptidase N/CD13 Antibody (ER-BMDM1) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10 - 1:50

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:10 - 1:500

Immunohistochemistry-Paraffin

1:10 - 1:500
Application Notes
For Flow Cytometry: Use 10 uL of the suggested working dilution to label 10^6 cells in 100 uL.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aminopeptidase N/CD13

Human CD13 is expressed on the majority of peripheral blood monocytes and granulocytes. It is also expressed by the majority of acute myeloid leukemias, chronic myeloid leukemias in myeloid blast crisis, a smaller percentage of lymphoid leukemias and myeloid cell lines. CD13 is also found in several types of non hematopoietic cells such as fibroblasts and endothelial cells and in a soluble form in blood plasma. CD13 is not expressed on B cells, T cells, platelets or erythrocytes. In the mouse, CD13 is a non-covalently linked homodimer of approximately 150kD subunits expressed by a variety of cells including monocytes, macrophages, dendritic cell and veiled cells. CD13 plays a role in biologically active peptide metabolism, in the control of growth and differentiation, in phagocytosis and in bactericidal/tumoricidal activities. CD13 also serves as a receptor for human coronaviruses (HCV).

Alternate Names

Aminopeptidase M, ANPEP, APN, CD13, gp150, PEPN

Entrez Gene IDs

16790 (Mouse)

Gene Symbol

ANPEP

UniProt

P97449

Additional Aminopeptidase N/CD13 Products

Product Documents for Aminopeptidase N/CD13 Antibody (ER-BMDM1) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Aminopeptidase N/CD13 Antibody (ER-BMDM1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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