Aquaporin-4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87679
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat, Bovine
Cited:
Human, Mouse, Rat, Porcine, Bovine
Applications
Validated:
Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Block/Neutralize, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Aquaporin-4 Antibody
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: CPDVEFKRRFKEAFSKAAQQTKGSYMEVEDNRSQVETDDLILKPGVVHVIDVDRGEEKKGKDQSGEVLSSV
Reactivity Notes
Use in Rat reported in scientific literature (PMID:33634832). Bovine reactivity reported in scientific literature (PMID: 31233960).
Marker
Astrocytes Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Aquaporin-4 Antibody
Western Blot: Aquaporin-4 Antibody [NBP1-87679] - Analysis in human brain tissue.
Immunocytochemistry/Immunofluorescence: Aquaporin-4 Antibody [NBP1-87679] - Staining of human cell line U-251 MG shows localization to plasma membrane. Antibody staining is shown in green.
Applications for Aquaporin-4 Antibody
Application
Recommended Usage
Immunocytochemistry/Immunofluorescence
0.25 - 2 ug/mL
Immunohistochemistry
1:2500 - 1:5000
Immunohistochemistry-Paraffin
1:2500 - 1:5000
Western Blot
0.04 - 0.4 ug/mL
Application Notes
Use in Immunoprecipitation reported in scientific literature (PMID: 32843684). WB reported in scientific literature (PMID: 26039099). Aquaporin-4 antibody validated for IHC-F, IHC-P from verified customer reviews. For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4.5 using NBP1-87679 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2), 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Aquaporin 4/AQP4
Long Name
Aquaporin 4
Alternate Names
AQP-4, AQP4, MIWC, WCH4
Entrez Gene IDs
361 (Human)
Gene Symbol
AQP4
UniProt
Additional Aquaporin 4/AQP4 Products
Product Documents for Aquaporin-4 Antibody
Product Specific Notices for Aquaporin-4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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