Aquaporin-4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12902
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Predicted:
Bovine (95%), Human (92%), Porcine (92%), Rhesus Macaque (95%), Sheep (95%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of mouse Aquaporin-4. The exact sequence is proprietary.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge briefly prior to opening.
Scientific Data Images for Aquaporin-4 Antibody
Western Blot: Aquaporin-4 Antibody [NBP3-12902]
Western Blot: Aquaporin-4 Antibody [NBP3-12902] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Aquaporin-4 antibody (NBP3-12902) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Immunocytochemistry/ Immunofluorescence: Aquaporin-4 Antibody [NBP3-12902]
Immunocytochemistry/Immunofluorescence: Aquaporin-4 Antibody [NBP3-12902] - Aquaporin-4 antibody detects Aquaporin-4 protein at astrocyte cells by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons and glia cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Aquaporin-4 protein stained by Aquaporin-4 antibody (NBP3-12902) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (NBP2-43561) diluted at 1:500. Blue: Fluoroshield with DAPI.Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP3-12902]
Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP3-12902] - Aquaporin-4 antibody detects Aquaporin-4 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Aquaporin-4 stained by Aquaporin-4 antibody (NBP3-12902) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 minApplications for Aquaporin-4 Antibody
Application
Recommended Usage
Immunocytochemistry/Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry Free-Floating
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol (pH7)
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Aquaporin 4/AQP4
Long Name
Aquaporin 4
Alternate Names
AQP-4, AQP4, MIWC, WCH4
Gene Symbol
AQP4
Additional Aquaporin 4/AQP4 Products
Product Specific Notices for Aquaporin-4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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