Brachyury Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24676
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Primate
Cited:
Human, Mouse
Predicted:
Canine (93%), Monkey (93%), Rat (93%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
ICC/IF, WB
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Summary for Brachyury Antibody
Immunogen
A portion of amino acids 350-400 of human Brachyury was used as the immunogen.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 28132888).
Marker
Mesoderm Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Brachyury Antibody
Western Blot: Brachyury Antibody [NBP2-24676] - Analysis of Brachyury in U87 cell lysate. Lane 1 shows pre-immune sera, lane 2 shows this antibody tested at 2 ug/mL.
Immunohistochemistry-Paraffin: Brachyury Antibody [NBP2-24676] - Analysis of Brachyury in FFPE human brain tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/mL.
Applications for Brachyury Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:20 - 1:1000
Immunohistochemistry-Paraffin
5 ug/mL
Western Blot
1 - 3 ug/mL
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Brachyury
Alternate Names
brachyury protein, MGC104817, Protein T, SAVA, T brachyury (mouse) homolog, T brachyury homolog, T Brachyury Transcription Factor, T, brachyury homolog (mouse), T-Box Transcription Factor T, TBXT, TF, TFT
Gene Symbol
T
UniProt
Additional Brachyury Products
Product Documents for Brachyury Antibody
Product Specific Notices for Brachyury Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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