CD47 Antibody (B6H12.2) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-31106
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
CyTOF-ready, Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
ICC/IF, IHC-P, IP, WB
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # B6H12.2
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This antibody was raised against CD47.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 30833751).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for CD47 Antibody (B6H12.2) - Azide and BSA Free
Immunocytochemistry/Immunofluorescence: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD47 Antibody [B6H12.2] (NBP2-31106) at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Western Blot: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Detection of CD47 expression on two hematological cancer cell lines, Jurkat and Raji. WB image submitted by a verified customer review.
Immunohistochemistry-Paraffin: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Human breast cancer tissue stained with CD47 Antibody (B6H12.2). IHC-P image submitted by a verified customer review.
Applications for CD47 Antibody (B6H12.2) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1 ug/mL
Immunohistochemistry-Paraffin
0.5 ug/mL
Western Blot
1:1000
Application Notes
This antibody is CyTOF ready.
Reviewed Applications
Read 3 reviews rated 4.7 using NBP2-31106 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CD47
Additional CD47 Products
Product Documents for CD47 Antibody (B6H12.2) - Azide and BSA Free
Product Specific Notices for CD47 Antibody (B6H12.2) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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