CD47 Antibody (BLR131H)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14713

Recombinant Monoclonal
Novus Biologicals, part of Bio-Techne
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NBP3-14713
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR131H

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD47 Antibodies »

Product Summary for CD47 Antibody (BLR131H)

Immunogen

residues 19-142 (ECD)

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for CD47 Antibody (BLR131H)

Western Blot: CD47 Antibody (BLR131H) [NBP3-14713] - Whole cell lysate (2 ug) from Jurkat, RKO, GaMG, U2OS, OVCAR-3, HeLa, HEK293T, and A-549 cells prepared using NETN lysis buffer. Rabbit anti-CD47 recombinant monoclonal antibody used at 1:1000. secondary HRP-conjugated goat anti-rabbit IgG . Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody.
Immunocytochemistry: CD47 Antibody (BLR131H) [NBP3-14713] - Whole cell lysate (2 ug) from Jurkat, RKO, GaMG, U2OS, OVCAR-3, HeLa, HEK293T, and A-549 cells prepared using NETN lysis buffer.
Immunohistochemistry: CD47 Antibody (BLR131H) [NBP3-14713] - Whole cell lysate (2 ug) from Jurkat, RKO, GaMG, U2OS, OVCAR-3, HeLa, HEK293T, and A-549 cells prepared using NETN lysis buffer. secondary Rabbit anti-CD47 recombinant monoclonal antibody used at 1:1000.
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Applications for CD47 Antibody (BLR131H)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Simple Western

1:10 - 1:250

Western Blot

1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: CD47

CD47 antigen, also known as integrin associated protein (IAP), has a very broad tissue distribution. CD47 is expressed on all hematopoietic cells, including leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines. There are approximately 50.000 CD47 molecules per erythrocyte. The glycoprotein is deficient in erythrocytes of the rare Rh null phenotype. CD47 is a heavily N glycosylated cell membrane glycoprotein of apparent molecular weight 47-52 kDa. It may play a role as a signaltransducer in the regulation of cation fluxes across cell membranes and in the chemotactic and adhesive interactions of leukocytes with endothelial cells.

Alternate Names

CD47, IAP, MER6, OA3

Gene Symbol

CD47

Additional CD47 Products

Product Documents for CD47 Antibody (BLR131H)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD47 Antibody (BLR131H)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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