CXCL12/SDF-1 Antibody (MM0211-9N26) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-12221

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Rat, Sheep

Cited:

Rat

Applications

Validated:

Block/Neutralize, CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

ELISA, IF

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MM0211-9N26

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all CXCL12/SDF-1 Antibodies »

Product Specifications

Immunogen

Human recombinant CXCL12/SDF-1

Reactivity Notes

Use in Rat reported in scientific literature (PMID:32197646).

Specificity

Detects human CXCL12 (both 1-alpha and 1 beta).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CXCL12/SDF-1 Antibody (MM0211-9N26) - Azide and BSA Free

Immunohistochemistry-Frozen: CXCL12/SDF-1 Antibody (MM0211-9N26) [NBP2-12221] - Staining in human uterus tissue.
Immunohistochemistry-Paraffin: CXCL12/SDF-1 Antibody (MM0211-9N26) [NBP2-12221] - CXCL12/SDF1 in sheep bone marrow. Image from verified customer review.
Immunohistochemistry-Paraffin: CXCL12/SDF-1 Antibody (MM0211-9N26) [NBP2-12221] - 10% Buffer formalin fixed and parffine embedded human placetal tissue section (4um) is subjected IHC staining using NBP2-12221. Tissue section was pretreated in citric buffer (pH 6.0) with microwave for antige retrieval before IHC is applied.

Applications for CXCL12/SDF-1 Antibody (MM0211-9N26) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:20-200

Immunohistochemistry

1:50-100

Immunohistochemistry-Paraffin

1:50-100

Western Blot

1:500-1:1000
Application Notes
This antibody is Cytof ready. IHC-F reported in a verified customer review. Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID:32184090). Use in ELISA reported in scientific literature (PMID:32197646).

Reviewed Applications

Read 2 reviews rated 5 using NBP2-12221 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CXCL12/SDF-1

The C-X-C or alpha chemokine family is characterized by a pair of cysteine residues separated by a single amino acid and primarily functions as chemoattractants for neutrophils. The C-X-C family includes IL-8, NAP-2, MSGA and stromal cell-derived factor-1, or SDF-1. SDF-1 was originally described as a pre-B cell stimulatory factor, but has now been shown to function as a potent chemoattractant for T cells and monocytes, but not neutrophils. Receptors for the C-X-C family are G protein-coupled, seven-pass, transmembrane domain proteins which include IL-8RA, IL-8RB and fusin (also designated LESTR or CXCR-4). Fusin is highly homologous to the IL-8 receptors, sharing 37% sequence identity at the amino acid level. The IL-8 receptors bind to IL-8, NAP-2 and MSGA, while fusin binds to its cognate ligand, SDF-1. Fusin has been identified as the major co-receptor for T-tropic HIV-1, and SDF-1 has been shown to inhibit HIV-1 infection. Six human SDF-1 isoforms exist due to alternative splicing of CXCL12, the gene encoding SDF-1. Three isoforms are known for mouse and rat.

Alternate Names

PBSF, SDF-1, SDF1

Gene Symbol

CXCL12

UniProt

Q6ICW0

Additional CXCL12/SDF-1 Products

Product Documents for CXCL12/SDF-1 Antibody (MM0211-9N26) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CXCL12/SDF-1 Antibody (MM0211-9N26) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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