DCLK1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36420
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Summary for DCLK1 Antibody
Immunogen
The immunogen this antibody was made to, maps to a region between residue 690 to 740 of human Doublecortin-Like and CAM Kinase-Like 1 using the numbering given in entry O15075.2 (GeneID 9201).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for DCLK1 Antibody
Western Blot: DCLK1 Antibody [NBP2-36420] - Detection of Human and Mouse DCAMKL1 by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-DCAMKL1 antibody NBP2-36420 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: DCLK1 Antibody [NBP2-36420] - Detection of human DCAMKL1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from 293T and cells. Antibodies: Affinity purified rabbit anti-DCAMKL1 antibody NBP2-36420 used for IP at 6 ug per reaction. DCAMKL1 was also immunoprecipitated by another rabbit anti-DCAMKL1 antibody. For blotting immunoprecipitated DCAMKL1, NBP2-36420 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Applications for DCLK1 Antibody
Application
Recommended Usage
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:500 - 1:2500
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: DCLK1
Long Name
Doublecortin-like and CAM Kinase-like 1
Alternate Names
CL1, CLICK1, DCAMKL1, DCDC3A
Gene Symbol
DCLK1
UniProt
Additional DCLK1 Products
Product Documents for DCLK1 Antibody
Product Specific Notices for DCLK1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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