ERK1 [p Thr202, p Tyr204] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB500-141
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Chicken
Cited:
Human, Chicken
Predicted:
Bovine (100%), Canine (100%), Primate (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IHC-P, WB
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding ERK1 conjugated to KLH. Accession # P63086
Reactivity Notes
Chicken reactivity reported in scientific literature (PMID: 31400551).
Modification
p Thr202, p Tyr204
Localization
Cell Membrane
Specificity
Specific for endogenous levels of the ~42 - 44 kDa ERK1 protein phosphorylated at Thr202 and Tyr204. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ERK1 [p Thr202, p Tyr204] Antibody
Western Blot: ERK1 [p Thr202, p Tyr204] Antibody [NB500-141] - Human T47D cell lysates showing specific immunolabeling of ~42-44k ERK/MAPK protein phosphorylated at Thr202/Tyr204 (Control). Phosphospecificity is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho Thr202/Tyr204 ERK/MAP antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.
Immunohistochemistry: ERK1 [p Thr202, p Tyr204] Antibody [NB500-141] - Immunostaining of granule cells in the dentate gyrus of saline treated mouse showing ERK/MAPK when phosphorylated at Thr202/Tyr204 (red) and nuclei (blue). Photo courtesy of Robert Wine.
Applications for ERK1 [p Thr202, p Tyr204] Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100 - 1:250
Immunohistochemistry
1:500
Western Blot
1:1000
Application Notes
NB500-141 can be used in Western blot where a band is seen at ~ 42-44 kDa, representing ERK 1/2. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 26232338)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ERK1
Long Name
Extracellular Signal-regulated Kinase 1
Alternate Names
MAPK3, P44ERK1, p44mapk, PRKM3
Gene Symbol
MAPK3
UniProt
Additional ERK1 Products
Product Documents for ERK1 [p Thr202, p Tyr204] Antibody
Product Specific Notices for ERK1 [p Thr202, p Tyr204] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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