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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for FGF-10 Antibody

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human FGF-10.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for FGF-10 Antibody

Western Blot: FGF-10 Antibody [NBP2-98997] - Lane A: A549 Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: NCI-H460 Whole Cell LysateLane D: Mouse lung tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:23 kDaObserved band size:42 kDa
Immunohistochemistry-Paraffin: FGF-10 Antibody [NBP2-98997] - Staining of human FGF-10 in human prostatic carcinoma with rabbit polyclonal antibody (1:1000).
Immunoprecipitation: FGF-10 Antibody [NBP2-98997] - Lane A:0.5 mg A549 Whole Cell LysateLane B:0.5 mg MCF-7 Whole Cell Lysate4 uL anti-FGF10 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-FGF10 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 23 kDaObserved band size: 42 kDa

Applications for FGF-10 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500-1:2000

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

4-8 uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FGF-10

The FGF10 gene encodes a 207 amino acid long, 23 kDA fibroblast growth factor 10 protein that is active in embryonic development, cell proliferation, and cell differentiation as it is a member of the fibroblast growth factor family (FGF). Additionally, the FGF10 gene functions in wound healing and is necessary for normal branching morphogenesis. FGF10 participates in regulation of actin cytoskeleton, mitochondrial apoptosis, paxillin interactions, nuclear receptor activation by vitamin-A, downstream signal transduction, and FGFR2 and FGFR ligand binding and activation. It is known to interact with genes FGF1, FGFR2, POMC, FGF18, and FGFBP1. FGF10 is linked to ladd syndrome, intestinal atresia, ectodermal dysplasia, craniosynostosis, cleft lip/palate, clear cell acanthoma, and gilles de la tourette syndrome.

Long Name

Fibroblast Growth Factor 10

Alternate Names

FGF10, KGF-2, KGF2

Gene Symbol

FGF10

Additional FGF-10 Products

Product Documents for FGF-10 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FGF-10 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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