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gamma Catenin Antibody ([11E4]) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54528

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # [11E4]

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant full length human plakoglobin, fused to maltose binding protein (Uniprot: P14923)

Localization

Cell Surface and Cytoplasmic

Marker

Cardiomyocyte Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53306).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Scientific Data Images for gamma Catenin Antibody ([11E4]) - Azide and BSA Free

Western Blot: gamma Catenin Antibody (11 E4) - Azide and BSA Free [NBP2-54528] - Western Blot Analysis of human HeLa and SKBR3 cell lysate using gamma Catenin Antibody (11 E4).
Immunocytochemistry/Immunofluorescence: gamma Catenin Antibody (11 E4) - Azide and BSA Free [NBP2-54528] - Confocal immunofluorescence image of HeLa cells using gamma Catenin Antibody (11 E4) Green (CF488) and Red Dot is used to label the nuclei Red.
Flow Cytometry: gamma Catenin Antibody (11 E4) - Azide and BSA Free [NBP2-54528] - Flow Cytometric Analysis of PFA-fixed MCF cells. gamma Catenin Antibody (11 E4) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Applications for gamma Catenin Antibody ([11E4]) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 - 1 ug/million cells

Immunocytochemistry/Immunofluorescence

1 - 2 ug/ml

Western Blot

0.5 - 1.0 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: gamma Catenin

Catenin gamma, also known as Junction Plakoglobin, is a common junctional plaque protein. It is a cytoplasmic protein with soluble and membrane-associated forms. The presence of Catenin gamma in both the desmosomes and in the intermediate junctions suggests it has a role in the structure and Function of submembranous plaques.

Alternate Names

catenin (cadherin-associated protein), gamma (80kD), catenin (cadherin-associated protein), gamma 80kDa, Catenin gamma, Desmoplakin III, Desmoplakin-3, DP3CTNNG, DPIII, gamma-catenin, junction plakoglobin, PDGBARVD12, PKGB

Gene Symbol

JUP

UniProt

Additional gamma Catenin Products

Product Documents for gamma Catenin Antibody ([11E4]) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for gamma Catenin Antibody ([11E4]) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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