gamma Catenin Antibody (9O2K8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16344
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 9O2K8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for gamma Catenin Antibody (9O2K8)
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 15-115 of human gamma Catenin (P14923). EWQQTYTYDSGIHSGANTCVPSVSSKGIMEEDEACGRQYTLKKTTTYTQGVPPSQGDLEYQMSTTARAKRVREAMCPGVSGEDSSLLLATQVEGQATNLQR
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for gamma Catenin Antibody (9O2K8)
Western Blot: gamma Catenin Antibody (9O2K8) [NBP3-16344] - Western blot analysis of extracts of various cell lines, using gamma Catenin Rabbit mAb (NBP3-16344) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunocytochemistry/Immunofluorescence: gamma Catenin Antibody (9O2K8) [NBP3-16344] - Immunofluorescence analysis of NIH-3T3 cells using gamma Catenin Rabbit mAb (NBP3-16344) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: gamma Catenin Antibody (9O2K8) [NBP3-16344] - Immunohistochemistry of paraffin-embedded mouse testis using gamma Catenin Rabbit mAb (NBP3-16344) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Applications for gamma Catenin Antibody (9O2K8)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: gamma Catenin
Alternate Names
catenin (cadherin-associated protein), gamma (80kD), catenin (cadherin-associated protein), gamma 80kDa, Catenin gamma, Desmoplakin III, desmoplakin-3, DP3CTNNG, DPIII, gamma-catenin, junction plakoglobin, PDGBARVD12, PKGB
Gene Symbol
JUP
Additional gamma Catenin Products
Product Documents for gamma Catenin Antibody (9O2K8)
Product Specific Notices for gamma Catenin Antibody (9O2K8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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