gamma Catenin Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1060
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Canine (100%), Porcine (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Summary for gamma Catenin Antibody
Immunogen
Peptide with sequence C-GLRPPYPTADHMLA corresponding to C-Terminus according to NP_002221.1, NP_068831.1.
Reactivity Notes
Predicted cross-reactivity based on sequence identity: Canine, Porcine.
Localization
Cytoplasmic in a soluble and membrane-associated form.
Specificity
This antibody is expected to recognise both reported isoforms, as represented by NP_002221.1 and NP_068831.1.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for gamma Catenin Antibody
Western Blot: gamma Catenin Antibody [NB100-1060] - Staining (1ug/ml) of Rat Eye lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry-Paraffin: gamma Catenin Antibody [NB100-1060] - Staining of Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: gamma Catenin Antibody [NB100-1060] - Staining of A431 (A) and NIH3T3 (B) cell lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Applications for gamma Catenin Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
5 ug/ml
Peptide ELISA
Detection limit 1:32000
Western Blot
0.1 - 0.3 ug/ml
Application Notes
EIA: An anonymous customer has reported successful usage quantitative ELISA assays (EIA), very sensitive to freeze/thaw cycles. WB: Approx. 85 kDa band observed in lysates of A431A 549 and NIH3T3 and in rat eye lysates (calculated MW of 81.7 kDa band according to human NP_002221.1 and mouse NP_034723.1).
IHC-Paraffin embedded Human Heart.
IHC-Paraffin embedded Human Heart.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: gamma Catenin
Alternate Names
catenin (cadherin-associated protein), gamma (80kD), catenin (cadherin-associated protein), gamma 80kDa, Catenin gamma, Desmoplakin III, Desmoplakin-3, DP3CTNNG, DPIII, gamma-catenin, junction plakoglobin, PDGBARVD12, PKGB
Gene Symbol
JUP
UniProt
Additional gamma Catenin Products
Product Documents for gamma Catenin Antibody
Product Specific Notices for gamma Catenin Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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