Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Chicken, Plant, Protozoa

Cited:

Human, Mouse

Applications

Validated:

Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Cited:

ICC/IF, IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # TU-30

Format

BSA Free

Concentration

1.0 mg/ml

View all gamma Tubulin Antibodies »

Product Summary for gamma Tubulin Antibody (TU-30) - BSA Free

Immunogen

C-terminal peptide of gamma-tubulin counjugated to KLH.

Epitope

The epitope was located in the amino acid sequence TRPDYI (aa439-444 in human), which is present on human gamma-tubulin 1 but not on human gamma-tubulin 2.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

The antibody TU-30 recognizes C-terminal peptide sequence EYHAATRPDYISWGTQ (aa 434-449) of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC).

Marker

Centrosome Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for gamma Tubulin Antibody (TU-30) - BSA Free

Western Blot: gamma Tubulin Antibody (TU-30) [NB500-574] - Western blotting analysis of differential reactivity of monoclonal antibodies to gamma-tubulin with human gamma-tubulin isotypes. (A) Immunoblots of total cell lysates from SH-SY5Y cells, expressing TagRFP-tagged human gamma-tubulin 1 (gamma-Tb1) or gamma-tubulin 2 (gamma-Tb2), probed with Abs to gamma-tubulin (TU-30, TU-32), TagRFP (RFP) and GAPDH. In control samples, only secondary anti-mouse Ab was applied. (B) Immunoblots of immobilized GST-tagged human C-terminal regions (a.a. 362-451) of gamma-Tb1 or gamma-Tb2 probed with Abs to gamma-tubulin (TU-30, TU-32) and GST. In control samples, only secondary anti-mouse Ab was applied.
Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody (TU-30) [NB500-574] - Staining of HeLa cells using anti-gamma Tubulin antibody (green). Nuclei were stained with DAPI (blue). Image from verified customer review.
Immunohistochemistry-Paraffin: gamma Tubulin Antibody (TU-30) - BSA Free [NB500-574] - FFPE Glioblastoma PDX is stained with mouse-gamma tubulin antibody (NB500-574) (red) for centrosomes and rabbit Arl13B (green) for primary cilia. Gamma Tubulin antibody is diluted 1:25, incubated overnight at 4C. Image from verified customer review.
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Applications for gamma Tubulin Antibody (TU-30) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Western Blot

1:1000
Application Notes
Immunocytochemistry - Staining technique: (a) Fix cells for 10 min in methanol at -20oC and for 6 min in acetone at -20oC; (b) Fix cells directly in methanol for 10 min at -20oC or in acetone for 10 min at -20oC. Incubation: 45 min in room temperature Application note: The antibody TU-30 stains only fixed cells. gamma Tubulin Antibody (TU-30) - BSA Free validated for Immunohistochemistry-Paraffin from a verified customer review.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using NB500-574 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: gamma Tubulin

The gamma-tubulin (TUBG1; relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01% of tubulin dimer). The gamma-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing gamma-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface.

Long Name

Tubulin gamma-1 chain

Alternate Names

Gamma-1-tubulin, GCP-1, TUBG, TUBG1

Gene Symbol

TUBG1

Additional gamma Tubulin Products

Product Documents for gamma Tubulin Antibody (TU-30) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for gamma Tubulin Antibody (TU-30) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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