GOLM1 Antibody

Knockout Validated: GOLM1 Antibody [NBP1-50627] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and GOLM1 knockout (KO) HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1:5,000 of Rabbit Anti-Human GOLM1 polyclonal Antibody (Catalog # NBP1-50627) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for GOLM1 at approximately 75 kDa (as indicated) in the parental HEK293 celll line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions.

Western Blot: GOLM1 Antibody [NBP1-50627] - Western Blot Image of GOLM1. Whole cell protein from HeLa (lane 1), 3T3 (lane 2), MEF (lane 3), Raw264.7 (lane 4), L929 (lane 5), Neuro2A (lane 6) and PC12 (lane 7) were separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 mg/ml anti-GOLM1 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Immunocytochemistry/Immunofluorescence: GOLM1 Antibody [NBP1-50627] - Confocal immunofluorescent analysis of HeLa cells using GOLM1 antibody (NBP1-50627, 1:25). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).