GSK-3 alpha/beta [p Tyr216] Antibody (M132)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23367

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # M132

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GSK-3 alpha/beta Antibodies »

Product Summary for GSK-3 alpha/beta [p Tyr216] Antibody (M132)

Immunogen

Clone M132 was generated from a phospho-GSK-3 beta (Tyr-216) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 216 of human GSK-3 beta. This peptide sequence is also found in GSK-3 alpha (Tyr-279) and is highly conserved in GSK-3 genes in rat and mouse. [UniProt# P49840]

Modification

p Tyr216

Specificity

The antibody detects 46/50 kDa proteins corresponding to the apparent molecular mass of GSK-3 alpha/beta on SDS-PAGE immunoblots of pervanadate treated rabbit fibroblasts, as well as treated human SKN-SH and A431 cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for GSK-3 alpha/beta [p Tyr216] Antibody (M132)

GSK-3 alpha/beta [p Tyr216] Antibody (M132)

Western Blot: GSK-3 alpha/beta [p Tyr216] Antibody (M132) [NBP3-23367] -

Western Blot: GSK-3 alpha/beta [p Tyr216] Antibody (M132) [NBP3-23367] - Western blot analysis of rabbit spleen fibroblasts serum starved for 2 hrs (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-GSK-3 beta (lanes 1 & 2) or anti-GSK-3 alpha/beta (Y279/Y216) (lanes 3 & 4).

Applications for GSK-3 alpha/beta [p Tyr216] Antibody (M132)

Application
Recommended Usage

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: GSK-3 alpha/beta

Glycogen Synthase Kinase 3beta (GSK-3beta) is a unique serine/threonine kinase that is inactivated by phosphorylation. In response to insulin binding, PKB/AKT phosphorylates GSK-3beta on serine 9, which prevents GSK-3beta from phosphorylating glycogen synthase. Unphosphorylated glycogen synthase is active and able to synthesize glycogen. GSK-3beta is also unique in that it requires a substrate that has been phosphorylated by a distinct kinase before it can phosphorylate the substrate. This phosphate priming mechanism explains why phosphorylation of serine 9 inactivates GSK-3beta. The phosphorylated serine binds to the GSK-3beta priming phosphate position and prevents binding of alternative substrates. In addition to insulin signaling, GSK-3beta participates in the Wnt signaling pathway, where it forms a complex with axin, beta-catenin and adenomatous polyposis coli (APC) protein. In the presence of Wnts, GSK-3beta is unable to phosphorylate beta-catenin, which leads to stabilization of beta-catenin. The Wnt pathway inactivates GSK-3beta via the proteins, Dishevelled and FRAT, which disrupt the interaction of GSK-3beta with axin, beta-catenin, and APC. Clinically, there is considerable interest in GSK-3beta inhibitors because they may mimic the effect of insulin or reduce the hyperphosphorylation of Tau that is observed in Alzheimer's Disease.

Long Name

Glycogen Synthase Kinase 3

Alternate Names

DKFZp686D0638, EC 2.7.11, EC 2.7.11.26, glycogen synthase kinase 3 alpha, glycogen synthase kinase-3 alpha, GSK-3 alpha

Gene Symbol

GSK3A

Additional GSK-3 alpha/beta Products

Product Documents for GSK-3 alpha/beta [p Tyr216] Antibody (M132)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GSK-3 alpha/beta [p Tyr216] Antibody (M132)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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