GSK-3 alpha/beta [p Ser21] Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05443

Novus Biologicals, part of Bio-Techne
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NBP3-05443
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GSK-3 alpha/beta Antibodies »

Product Summary for GSK-3 alpha/beta [p Ser21] Antibody - Azide and BSA Free

Immunogen

A synthetic phosphorylated peptide around S21 of human GSK-3 alpha/beta (NP_063937.2). TSSFA

Modification

p Ser21

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GSK-3 alpha/beta [p Ser21] Antibody - Azide and BSA Free

Western Blot: GSK-3 alpha/beta [p Ser21] Antibody [NBP3-05443] - Western blot analysis of extracts of 293 cells, using GSK-3 alpha/beta antibody (NBP3-05443) at 1:1000 dilution or GSK3alpha antibody (A0645).293 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
Western Blot: GSK-3 alpha/beta [p Ser21] Antibody [NBP3-05443] - Western blot analysis of extracts of 293 and HeLa cells, using GSK-3 alpha/beta antibody (NBP3-05443) at 1:1000 dilution. 293 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. HeLa cells were treated by Insulin (0.01U/mL) for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
Immunoprecipitation: GSK-3 alpha/beta [p Ser21] Antibody [NBP3-05443] - Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug GSK-3 alpha/beta pAb (NBP3-05443). Western blot was performed from the immunoprecipitate using GSK-3 alpha/beta pAb (NBP3-05443) at a dilution of 1:1000. 293T cells were treated by Insulin (100 nM) at 37C for 10 minutes after serum-starvation overnight.

Applications for GSK-3 alpha/beta [p Ser21] Antibody - Azide and BSA Free

Application
Recommended Usage

Immunoprecipitation

1:50-1:100

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

Azide and BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GSK-3 alpha/beta

Glycogen Synthase Kinase 3beta (GSK-3beta) is a unique serine/threonine kinase that is inactivated by phosphorylation. In response to insulin binding, PKB/AKT phosphorylates GSK-3beta on serine 9, which prevents GSK-3beta from phosphorylating glycogen synthase. Unphosphorylated glycogen synthase is active and able to synthesize glycogen. GSK-3beta is also unique in that it requires a substrate that has been phosphorylated by a distinct kinase before it can phosphorylate the substrate. This phosphate priming mechanism explains why phosphorylation of serine 9 inactivates GSK-3beta. The phosphorylated serine binds to the GSK-3beta priming phosphate position and prevents binding of alternative substrates. In addition to insulin signaling, GSK-3beta participates in the Wnt signaling pathway, where it forms a complex with axin, beta-catenin and adenomatous polyposis coli (APC) protein. In the presence of Wnts, GSK-3beta is unable to phosphorylate beta-catenin, which leads to stabilization of beta-catenin. The Wnt pathway inactivates GSK-3beta via the proteins, Dishevelled and FRAT, which disrupt the interaction of GSK-3beta with axin, beta-catenin, and APC. Clinically, there is considerable interest in GSK-3beta inhibitors because they may mimic the effect of insulin or reduce the hyperphosphorylation of Tau that is observed in Alzheimer's Disease.

Long Name

Glycogen Synthase Kinase 3

Alternate Names

DKFZp686D0638, EC 2.7.11, EC 2.7.11.26, glycogen synthase kinase 3 alpha, glycogen synthase kinase-3 alpha, GSK-3 alpha

Gene Symbol

GSK3A

Additional GSK-3 alpha/beta Products

Product Documents for GSK-3 alpha/beta [p Ser21] Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GSK-3 alpha/beta [p Ser21] Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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