HNF-3 beta/FoxA2 Antibody (6C12)
Novus Biologicals, part of Bio-Techne | Catalog # H00003170-M12
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
FLOW, ICC/IF, IF, WB
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 6C12
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for HNF-3 beta/FoxA2 Antibody (6C12)
Immunogen
FOXA2 (NP_068556, 363 a.a. ~ 457 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. HLKPEHHYAFNHPFSINNLMSSEQQHHHSHHHHQPHKMDLKAYEQVMHYPGYGSPMPGSLAMGPVTNKTGLDASPLAADTSYYQGVYSRPIMNSS
Specificity
forkhead box A2
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for HNF-3 beta/FoxA2 Antibody (6C12)
Western Blot: HNF-3 beta/FoxA2 Antibody (6C12) [H00003170-M12] - Analysis of FOXA2 expression in K-562 (Cat # L009V1).
Immunocytochemistry/Immunofluorescence: HNF-3 beta/FoxA2 Antibody (6C12) [H00003170-M12] - Analysis of monoclonal antibody to FOXA2 on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: HNF-3 beta/FoxA2 Antibody (6C12) [H00003170-M12] - Analysis of monoclonal antibody to FOXA2 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 0.5 ug/ml
Applications for HNF-3 beta/FoxA2 Antibody (6C12)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:500
Application Notes
Use in Flow Cytometry reported in scientific literature (PMID:34280890). Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: HNF-3 beta/FoxA2
Long Name
Hepatocyte Nuclear Factor-3, beta
Alternate Names
FoxA2, HNF3 beta
Entrez Gene IDs
3170 (Human)
Gene Symbol
FOXA2
OMIM
600288 (Human)
UniProt
Additional HNF-3 beta/FoxA2 Products
Product Documents for HNF-3 beta/FoxA2 Antibody (6C12)
Product Specific Notices for HNF-3 beta/FoxA2 Antibody (6C12)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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