HNF-3 beta/FoxA2 Antibody (OTI3C10)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02088
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
ICC/IF, WB
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI3C10
Concentration
1 mg/ml
Product Summary for HNF-3 beta/FoxA2 Antibody (OTI3C10)
Immunogen
Recombinant protein expressed in E.coli corresponding to amino acids 300-458 of human FOXA2
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
48.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for HNF-3 beta/FoxA2 Antibody (OTI3C10)
Western Blot: HNF-3 beta/FoxA2 Antibody (OTI3C10) [NBP2-02088] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti HNF-3 beta/FoxA2 monoclonal antibody.(1:200).
Immunocytochemistry/Immunofluorescence: HNF-3 beta/FoxA2 Antibody (OTI3C10) [NBP2-02088] - Staining of HepG2 cells using anti HNF-3 beta/FoxA2 mouse monoclonal antibody at 1:50 dilution.
Immunohistochemistry-Paraffin: HNF-3 beta/FoxA2 Antibody (OTI3C10) [NBP2-02088] - Staining of paraffin-embedded lung using anti-FOXA2 mouse monoclonal antibody.
Applications for HNF-3 beta/FoxA2 Antibody (OTI3C10)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:50-1:100
Immunohistochemistry
1:50
Immunohistochemistry-Paraffin
1:50
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HNF-3 beta/FoxA2
Long Name
Hepatocyte Nuclear Factor-3, beta
Alternate Names
FoxA2, HNF3 beta
Entrez Gene IDs
3170 (Human)
Gene Symbol
FOXA2
Additional HNF-3 beta/FoxA2 Products
Product Documents for HNF-3 beta/FoxA2 Antibody (OTI3C10)
Product Specific Notices for HNF-3 beta/FoxA2 Antibody (OTI3C10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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