Human CD47 APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB4670A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 472603
Product Summary for Human CD47 APC-conjugated Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD47
Gln19-Pro139
Accession # Q08722
Gln19-Pro139
Accession # Q08722
Specificity
Detects human CD47 in flow cytometry. Stains human CD47 transfectants but not irrelevant transfectants.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human CD47 APC-conjugated Antibody
Detection of CD47 in Human Blood Lymphocytes by Flow Cytometry.
Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD47 APC-conjugated Monoclonal Antibody (Catalog # FAB4670A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human CD47 APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Human peripheral blood lymphocytes
Sample: Human peripheral blood lymphocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: CD47
CD47, also known as Integrin-associated Protein (IAP), is a ubiquitous 50 kDa multipass transmembrane protein with a single V-type Ig-like domain at its N-terminus. CD47 binding to SIRP alpha prevents the phagocytic engulfment of viable cells. It also binds to VEGFR2, inducing VEGFR2 signaling in both T cells and endothelial cells. Thrombospondin interaction with CD47 on T cells reduces T cell proliferation and inflammatory reactions. Alternative splicing generates isoforms with truncated cytoplasmic domains. Within the N-terminal ECD, human CD47 shares 63% aa sequence identity with mouse and rat CD47.
Alternate Names
CD47, IAP, MER6, OA3
Entrez Gene IDs
Gene Symbol
CD47
UniProt
Additional CD47 Products
Product Documents for Human CD47 APC-conjugated Antibody
Product Specific Notices for Human CD47 APC-conjugated Antibody
For research use only
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