Detects human Integrin alpha2b/CD41 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Integrin alpha5, alpha8, alphaV, beta3, beta5, beta6, recombinant mouse Integrin beta2b or beta3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human Integrin alpha2b/CD41 Antibody

Detection of Human Integrin alpha2b/CD41 by Western Blot.

Western blot shows lysates of human platelets. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Integrin a2b/CD41 Monoclonal Antibody (Catalog # MAB7616) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for Integrin a2b/CD41 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.

Detection of Integrin alpha2b/CD41 in Human Blood Platelets by Flow Cytometry.

Human peripheral blood platelets were stained with Fluorescein-conjugated Anti-Mouse Integrin a2b/CD41 (Clone P2) and either (A) Mouse IgG₁Isotype Control (MAB002) or (B) Mouse Anti-Human Integrin a2b/CD41 Mono-clonal Antibody (Catalog # MAB7616) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). MAB7616 stains the same population as Clone P2.

Detection of Integrin alpha 2b/CD41 in Human Blood Platelets by Flow Cytometry.

Human peripheral blood platelets were stained with Mouse Anti-Human Integrin a2b/CD41 Mono-clonal Antibody (Catalog # MAB7616, filled histogram) or Mouse IgG1 Isotype Control (MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Integrin alpha2b/CD41 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood platelets

Western Blot

2 µg/mL
Sample: Human platelets

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB7616 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 2b/CD41

Integrin alpha2b (ITGA2b), also known as CD41 and GPIIb, is a transmembrane glycoprotein that is expressed by megakaryocytes and platelets. It is cleaved into two disulfide-linked chains (114 kDa and 22 kDa) during transit through the Golgi. Integrin alpha2b associates with Integrin beta3 to form complexes that interact with Fibrinogen, von Willebrand factor, Fibronectin, and Vitronectin. Integrin alpha2b is required for platelet aggregation, and defects lead to disorders of coagulation. Within the extracellular domain, human Integrin alpha2b shares 80% and 78% amino acid sequence identity with mouse and rat Integrin alpha2b, respectively.

Alternate Names

CD41, GP2B, GPIIb, GTA, HPA3, ITGA2b

Entrez Gene IDs

3674 (Human); 16399 (Mouse); 29350 (Rat); 102119413 (Cynomolgus Monkey)

Gene Symbol

ITGA2B

Additional Integrin alpha 2b/CD41 Products

Product Documents for Human Integrin alpha2b/CD41 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human Integrin alpha2b/CD41 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Integrin alpha2b/CD41 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7616

Key Product Details

Species Reactivity

Validated:

Cited:

Applications