Human/Rat GFAP Antibody

Detection of Human and Rat GFAP by Western Blot.

Western blot shows lysates of rat cortical stem cells, rat brain tissue, human brain (cortex) tissue, and human brain (hypothalamus) tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2594) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for GFAP at approximately 35-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

beta-III Tubulin in Rat Cortical Neurons and GFAP in Rat Astrocytes.

beta-III Tubulin was detected in rat cortical neurons using 5 µg/mL Mouse Anti-neuron-specific Mouse beta-III Tubulin Monoclonal (clone TuJ-1) Antibody (Catalog # MAB1195). GFAP was detected in rat astrocytes using 10 µg/mL Sheep Anti-Human GFAP Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF2594). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for beta-III Tubulin using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and for GFAP using the Northern-Lights 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

GFAP in Rat Astrocytes.

GFAP was detected in immersion fixed rat astrocytes using 10 µg/mL Sheep Anti-Human GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2594) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.