Human SOX2 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF2018
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Flow Cytometry, ICC, IHC
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Summary for Human SOX2 Biotinylated Antibody
Immunogen
E. coli-derived recombinant human SOX2
Gly135-Met317
Accession # P48431
Gly135-Met317
Accession # P48431
Specificity
Detects SOX2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) SOX17 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human SOX2 Biotinylated Antibody
SOX2 in Human iPSK3 cells.
SOX2 was detected in immersion fixed human plasmid-derived induced pluripotent stem cells (iPSK3) using Goat Anti-Human SOX2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.Applications for Human SOX2 Biotinylated Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed NTera-2 human testicular embryonic carcinoma cell line and human plasmid-derived induced pluripotent stem cells (iPSK3)
Sample: Immersion fixed NTera-2 human testicular embryonic carcinoma cell line and human plasmid-derived induced pluripotent stem cells (iPSK3)
Western Blot
0.1 µg/mL
Sample: Recombinant Human SOX2
Sample: Recombinant Human SOX2
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOX2
References
- Graham, V. et al. (2003) Neuron 39:749.
- Avilion, A.A. et al. (2003) Genes Dev. 17:126.
- Kishi, M. et al. (2000) Development 127:791.
- Yuan, H. et al. (1995) Genes Dev. 9:2635.
- Uwanogho, D. et al. (1995) Mech. Dev. 49:23.
- Stevanovic, M. (2003) Mol. Biol. Rep. 30:127.
Long Name
Transcription Factor SOX2
Alternate Names
ANOP3, MCOPS3, MGC2413, SRY (sex determining region Y)-box 2, SRY-related HMG-box gene 2, transcription factor SOX2, transcription factor SOX-2
Entrez Gene IDs
6657 (Human)
Gene Symbol
SOX2
UniProt
Additional SOX2 Products
Product Documents for Human SOX2 Biotinylated Antibody
Product Specific Notices for Human SOX2 Biotinylated Antibody
For research use only
Loading...
Loading...
Loading...
Loading...