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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2D8

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Summary for Islet-1 Antibody (OTI2D8) - Azide and BSA Free

Immunogen

Human recombinant protein fragment corresponding to amino acids 134-349 of human ISL1(NP_002193) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

38.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Islet-1 Antibody (OTI2D8) - Azide and BSA Free

Western Blot: Islet-1 Antibody (OTI2D8) - Azide and BSA Free [NBP2-71050] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Islet-1.

Applications for Islet-1 Antibody (OTI2D8) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Islet-1

Islet 1 encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young.

Alternate Names

ISL1, Islet1

Gene Symbol

ISL1

Additional Islet-1 Products

Product Documents for Islet-1 Antibody (OTI2D8) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Islet-1 Antibody (OTI2D8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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