LSD1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-536
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation Sequencing, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Summary for LSD1 Antibody
Immunogen
The immunogen recognized by this antibody maps to a region between residues 50 and 100 of human FAD-binding protein BRAF35-HDAC complex 110 kDa using the numbering given in TrEMBL entry O60341 (GeneID 23028).
Specificity
NB100-536 is specific for human BHC110-KIAA0601 protein.
Marker
Nucleus Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for LSD1 Antibody
Western Blot: LSD1 Antibody [NB100-536] - Detection of human LSD1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-LSD1 antibody NB100-536 (lot NB100-536-2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. LSD1 was also immunoprecipitated by a previous lot (lot NB100-536-1) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Immunohistochemistry-Paraffin: LSD1 Antibody [NB100-536] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-BHC110/LSD1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: LSD1 Antibody [NB100-536] - Detection of Human BHC110/KIAA0601 by Western Blot. Samples: Nuclear extract (5 and 20 ug) from HeLa cells. Antibody: NB100-536 used at 0.01 ug/ml. Detection: Chemiluminescence with an exposure time of 15 minutes.
Applications for LSD1 Antibody
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
10 ug
Immunohistochemistry
1:500 - 1:2000
Immunohistochemistry-Paraffin
1:500 - 1:2000
Immunoprecipitation
2 - 5 ug/mg lysate
Western Blot
1:5000-1:15000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Lysine (K)-specific Demethylase 1A/KDM1A/LSD1
Alternate Names
AOF2, BHC110, LSD1, Lysine (K)specific Demethylase 1A
Gene Symbol
KDM1A
UniProt
Additional Lysine (K)-specific Demethylase 1A/KDM1A/LSD1 Products
- All Products for Lysine (K)-specific Demethylase 1A/KDM1A/LSD1
- Lysine (K)-specific Demethylase 1A/KDM1A/LSD1 Activity Assays
- Lysine (K)-specific Demethylase 1A/KDM1A/LSD1 Lysates
- Lysine (K)-specific Demethylase 1A/KDM1A/LSD1 Primary Antibodies
- Lysine (K)-specific Demethylase 1A/KDM1A/LSD1 Proteins and Enzymes
Product Documents for LSD1 Antibody
Product Specific Notices for LSD1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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