Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MM0028-5D11

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all MMP-15/MT2-MMP Antibodies »

Product Summary for MMP-15/MT2-MMP Antibody (MM0028-5D11) - Azide and BSA Free

Immunogen

Human recombinant MMP-15/MT2-MMP

Specificity

This antibody recognizes human MMP-15 in western blot without cross reactivity with human MMP-14/16.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for MMP-15/MT2-MMP Antibody (MM0028-5D11) - Azide and BSA Free

Immunohistochemistry-Paraffin: MMP15 Antibody (MM0028-5D11) [NB110-60994] - Human placenta (formalin fixed and paraffin embedded) section is subjected to IHC.

Applications for MMP-15/MT2-MMP Antibody (MM0028-5D11) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:400-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.2 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MMP-15/MT2-MMP

The mammalian matrix metalloproteinases (MMPs) degrade extracellular matrix in physiological and pathological processes. Of the sixteen members of matrix metalloproteinase family ten exist in soluble form whereas MT MMPs exist as integral membrane proteins. MMP15, first described as MTMMP2, is a metalloproteinase with a type I transmembrane domain. Initially reported in lung tissue, MMP15 message was also seen in testis, intestines, colon, heart, liver, kidney, pancreas and muscle. Western blots showed bands at 72 kD, 48 kD and 44 kD. Subsequent publications have focused on message, and found MMP15 in a wide range of tumor cell lines, as well as the synovial membrane from patients with rheumatoid arthritis. MMP15 is usually found in much lower levels than the more ubiquitous MMP14 (MT1MMP). MMP15 may be shed into the culture media when activated, but it is unclear if the shed form is proteolytically active. Cell lysates are the best source for Western blotting of MMP15.

Long Name

Matrix Metalloproteinase 15/Membrane Type 2 MMP

Alternate Names

MMP15, MT2-MMP

Entrez Gene IDs

4324 (Human)

Gene Symbol

MMP15

Additional MMP-15/MT2-MMP Products

Product Documents for MMP-15/MT2-MMP Antibody (MM0028-5D11) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MMP-15/MT2-MMP Antibody (MM0028-5D11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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