Mouse CD161/NK1.1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB76141
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Applications
CyTOF-ready, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # PK136
Product Summary for Mouse CD161/NK1.1 Antibody
Immunogen
NK-1+ cells from mouse spleen and bone marrow
Accession # AAH61168
Accession # AAH61168
Specificity
Detects mouse CD161/NK1.1 in Flow Cytometry.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Mouse CD161/NK1.1 Antibody
Detection of CD161/NK1.1 in Mouse Splenocytes by Flow Cytometry.
Mouse C57BL/6 splenocytes were stained with Mouse Anti-Mouse CD161/NK1.1 Monoclonal Antibody (Catalog # MAB76141, filled histogram) or Mouse IgG2A Isotype Control (Catalog # MAB003, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Histograms were gated on Rat Anti-Mouse NKp46/NCR1 PE-conjugated Monoclonal Antibody (Catalog # FAB22252P) positive cells. View our protocol for Staining Membrane-associated Proteins.Applications for Mouse CD161/NK1.1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: C57BL/6 mouse splenocytes
Sample: C57BL/6 mouse splenocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD161/NK1.1
NK-1.1 surface antigen is encoded by the NKR-P1B/NKR-P1C gene, also known as CD161b/CD161c and Ly-55. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN- gamma production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers. The PK136 antibody has been reported to deplete NK cells, induce redirected lysis, block NK cell function, and induce NK cell proliferation.
Additional CD161/NK1.1 Products
Product Documents for Mouse CD161/NK1.1 Antibody
Product Specific Notices for Mouse CD161/NK1.1 Antibody
For research use only
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