Mouse CD90/Thy1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF7335

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
AF7335-SP
AF7335
100% Guarantee

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all CD90/Thy1 Antibodies »

Product Specifications

Specificity

Detects mouse CD90/Thy1 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human CD90/Thy1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse CD90/Thy1 Antibody

Detection of Mouse CD90/Thy1 antibody by Western Blot.

Detection of Mouse CD90/Thy1 by Western Blot.

Western blot shows lysates of mouse thymus tissue, mouse brain tissue, EL-4 mouse lymphoblast cell line, and CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Mouse CD90/Thy1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7335) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD90/Thy1 at approximately 25-35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse CD90/Thy1 Antibody

Application
Recommended Usage

Western Blot

0.2 µg/mL
Sample: Mouse thymus tissue, mouse brain tissue, EL‑4 mouse lymphoblast cell line, and CTLL‑2 mouse cytotoxic T cell line

Formulation, Preparation, and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: CD90/Thy1

CD90, also known as Thy1 (THYmocyte differentiation antigen 1) is a GPI-linked glycoprotein expressed by endothelial cells, hematopoietic stem cells, and in developing nervous tissue. Unlike human CD90, mouse CD90 is also highly expressed on T cells and is involved in T cell activation. CD90 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

Alternate Names

CD90, Thy1

Entrez Gene IDs

7070 (Human); 21838 (Mouse)

Gene Symbol

THY1

UniProt

P01831 (Mouse)

Additional CD90/Thy1 Products

Product Documents for Mouse CD90/Thy1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse CD90/Thy1 Antibody

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.