Mouse/Rat Nestin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2736
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Cited:
Human, Mouse, Rat, Primate
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 307501
Product Summary for Mouse/Rat Nestin Antibody
Specificity
Detects mouse and rat Nestin in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Mouse/Rat Nestin Antibody
Nestin in Rat Cortical Stem Cells.
Nestin was detected in immersion fixed undifferentiated rat cortical stem cells using Mouse Anti-Rat Nestin Monoclonal Antibody (Catalog # MAB2736) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Nestin in C2C12 Mouse Cell Line.
Nestin was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Mouse/Rat Nestin Monoclonal Antibody (Catalog # MAB2736) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Mouse/Rat Nestin Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed undifferentiated rat cortical stem cells and C2C12 mouse myoblast cell line
Sample: Immersion fixed undifferentiated rat cortical stem cells and C2C12 mouse myoblast cell line
Western Blot
1 µg/mL
Sample: Recombinant Rat Nestin
Sample: Recombinant Rat Nestin
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 4 reviews rated 4.8 using MAB2736 in the following applications:
Formulation, Preparation, and Storage
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: Nestin
References
- Hockfield, S. and R.D. McKay (1985) J. Neurosci. 5:3310.
- Lendahl, U. et al. (1990) Cell 60:585.
- Frederiksen, K. and R.D. McKay (1988) J. Neurosci. 8:1144.
- Tohyama, T. et al. (1992) Lab. Invest. 66:303.
- Uchida, N. et al. (2000) Proc. Natl. Acad. Sci. USA 97:14720.
- Frederiksen, K. et al. (1988) Neuron 1:439.
- Cattaneo, E. et al. (1990) Nature 347:762.
- Reynolds, B.A. and S. Weiss (1992) Science 255:1707.
- Rietze, R.L. et al. (2001) Nature 412:736.
- Carpenter, M.K. et al. (2001) Exp. Neurol. 172:383.
- Zulewski, H. et al. (2001) Diabetes 50:521.
- Lumelsky, N. et al. (2001) Science 292:1389.
- Lechner, A. et al. (2001) Biochem. Biophys. Res. Commun. 293:670.
- Shih, C.C. et al. (2001) Blood 98:2412.
Alternate Names
NES
Gene Symbol
NES
UniProt
Additional Nestin Products
Product Documents for Mouse/Rat Nestin Antibody
Product Specific Notices for Mouse/Rat Nestin Antibody
For research use only
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