MUC5AC Antibody (45M1)

Immunohistochemistry-Paraffin: MUC5AC Antibody (45M1) [NBP2-15196] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC5AC Monoclonal Antibody (45M1).

Immunohistochemistry: MUC5AC Antibody (45M1) [NBP2-15196] -

Conditional deletion of Nedd4-2 in lung epithelial cells causes peripheral airway remodeling with increased Muc5b production.a-d Representative micrographs and high magnification insets illustrating the airway cell population of terminal bronchioles from conditional Nedd4-2-/- mice and littermate controls after 3 months of doxycycline induction stained with Alcian blue and periodic acid-Schiff (AB-PAS) (control, n = 5 mice; Nedd4-2-/-, n = 9 mice) (a), or co-stained with anti-Muc5b and anti-acetylated alpha-tubulin antibodies (control, n = 5 mice; Nedd4-2-/-, n = 6) (b, c), or with anti-Muc5ac and anti-CCSP antibodies (n = 4/group) (d). Scale bars, 60 and 15 um (insets). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32332792), licensed under a CC-BY licence.

Western Blot: MUC5AC Antibody (45M1) [NBP2-15196] -

UroA/UAS03 enhance tight junction proteins in AhR-dependent manner. a HT29 cells were treated with vehicle (DMSO-0.01%)/UroA/UAS03 (50 μM) for 24 h. mRNA levels of Cytochrome P450 1A1 (Cyp1A1) was measured by RT PCR. b Cyp1A1 protein levels were measured using immunoblots and quantified band intensities by Image J software. c Cyp1A1 enzyme activity was measured by P450-Glo Cyp1A1 assay. HT29 cells were treated with UroA or UAS03 (0.1, 1, 10, 25, 50 µM) or FICZ (0.1, 1, 10, 25, 50 nM) for 24 h and enzyme Cyp1A1 activity was measured. d C57BL/6 and AhR−/− (n = 3) mice were treated orally with Vehicle (0.25% CMC), UroA or UAS03 (20 mg/kg) for 1 week and Cyp1A1 activity was measured in colons and livers by ethoxyresorufin-O-deethylase (EROD) assay. e The cells expressing AhR-reporter (luciferase) were treated with Veh or UroA/UAS03 or ellagic acid (EA) or MeBio (AhR high affinity ligand) for 6 h and fold change of luminescence over vehicle treatment was measured. f Immunofluorescence confocal images of HT29 cells treated with vehicle/UroA/UAS03 (50 μM) for 6 h. The cells were stained with anti-AhR antibody (red) and DAPI (blue). Relative fluorescence (n = ~20 cells) in the cytosol and nucleus was measured. The scale bar indicates 10 μm. g AhR levels in cytosol and nuclear fractions of HT29 cells treated for 2 h with Veh or UroA/UAS03 (50 μM). h AhR or i Cyp1A1 was knocked down using siRNA in HT29 cells and the cells were treated with vehicle/UroA/UAS03 (50 μM) for 24 h and immnunoblots were performed to detect expression of AhR, Cyp1A1, and Cldn4. Scrambled (Sc) siRNA transfections were used as controls. Immunoblots were quantified using Image J software. The data is representative of two independent repeats with triplicate wells for each treatment. Statistics performed using unpaired t-test using Graphpad Prism software. All in vitro studies were performed in triplicates. Error bars, ±SEM; ***p < 0.001; **p < 0.01; **p < 0.05. Source Data are provided as a Source Data File Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30626868), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: MUC5AC Antibody (45M1) [NBP2-15196] -

RECQ5 promotes SUMO2 conjugation of PCNA. a Western blot analysis of RECQ5 in whole cell extracts (WCE) prepared from control or RECQ5 siRNA knockdown HEK293T cells treated with or without camptothecin (CPT, 5 µM). Actin was used as a loading control. b Western blot analysis of PCNA in the CB:RNA+ and CB:RNA− fractions prepared from HEK293T cells with or without CPT treatment from a. c Western blot analysis of the indicated proteins in CB:RNA+ and CB:RNA− fractions prepared from HEK293T cells with or without exogenous overexpression of FLAG-RECQ5. MCM7 was used as a loading control. d Western blot analysis of the indicated proteins in the input of the CB:RNA+ and CB:RNA− fractions with or without exogenous overexpression of FLAG-RECQ5 visualized with short and long exposure times (top) and in the FLAG-RECQ5 complex purified from the CB:RNA+ and CB:RNA− fractions shown above (bottom). e Amino acid sequences of the PCNA-Interacting-Protein (PIP) and PIP-like (PIP-L) motifs found in human RECQ5 protein are shown in bold. f Western blot analysis of the indicated proteins in WCE prepared from HEK293T cells with or without exogenous overexpression of FLAG-RECQ5 WT and mutant proteins. g Western blot analysis of the indicated proteins in the chromatin-bound (CB) fractions prepared from HEK293T cells from f. h Western blot analysis of the indicated proteins in the FLAG-RECQ5 complexes purified from CB fractions shown in g. i Western blot analysis of the indicated proteins in WCE prepared from control or RECQ5 siRNA knockdown HEK293T cells with exogenous overexpression of indicated FLAG-tagged PCNA constructs or CAF1 or treated with an empty vector (V). j Quantification of gammaH2AX-positive cells indicated in i. Each value in the graph represents the average value ± standard deviation (n > 200) per one representative experiment. Only cells with 5 or more gammaH2AX foci were counted as positive cells. p values equal to or less than 0.05 are indicated with an asterisk (*).The result was reproduced in two independent assays Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30006506), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: MUC5AC Antibody (45M1) [NBP2-15196] -

Ddx19 binds directly to MKL1 RPEL domain(a) Recruitment of Ipo beta and Ddx19 from Ipo beta-depleted NIH 3T3 cytoplasmic lysates by GST-RPEL. (−), NIH 3T3 cytoplasmic lysate transfected with control siRNAs; (+), Ipo beta-depleted NIH 3T3 cytoplasmic lysate. Ponceau-stained full membrane (below). (b) Purified his-tagged Ddx19 (+; 54 kDa right lane) binds directly to GST-RPEL (middle lane) but not to GST alone (left lane). Proteins are stained with Coomassie brilliant blue. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/ncomms6978), licensed under a CC-BY license. Not internally tested by Novus Biologicals.