NGFR/TNFRSF16/p75NTR Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85769

Novus Biologicals, part of Bio-Techne
Catalog #
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NBP1-85769
NBP1-85769-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (93%), Rat (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NGFR/TNFRSF16 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NKQGANSRPVNQTPPPEGEKLHSDSGISVDSQSLHDQQPHTQTASGQALKGDGGLYSSLPPAKREEVEKLLNGSAGDTWRHLAGELGYQPEHIDSFTHEACPVRALLASWATQDSATLDALLAALRRIQRADLVESLCSES

Reactivity Notes

Reactivity reported in scientific literature (PMID: 20184585).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NGFR/TNFRSF16/p75NTR Antibody

Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody [NBP1-85769]

Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody [NBP1-85769]

Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody [NBP1-85769] - Staining in human spleen and skeletal muscle tissues using NBP1-85769 antibody. Corresponding NGFR RNA-seq data are presented for the same tissues.
Western Blot: NGFR/TNFRSF16/p75NTR Antibody [NBP1-85769]

Western Blot: NGFR/TNFRSF16/p75NTR Antibody [NBP1-85769]

Western Blot: NGFR/TNFRSF16/p75NTR Antibody [NBP1-85769] - Analysis in human cell line RH-30.
Immunocytochemistry/ Immunofluorescence: NGFR/TNFRSF16/p75NTR Antibody [NBP1-85769]

Immunocytochemistry/ Immunofluorescence: NGFR/TNFRSF16/p75NTR Antibody [NBP1-85769]

Immunocytochemistry/Immunofluorescence: NGFR/TNFRSF16/p75NTR Antibody [NBP1-85769] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane. Antibody staining is shown in green.
View 5 more images

Applications for NGFR/TNFRSF16/p75NTR Antibody

Application
Recommended Usage

Immunocytochemistry/Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500-1:1000

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NGFR/TNFRSF16

p75NTR was originally discovered as a low affinity nerve growth factor receptor. Later it was found that it was the receptor for all neurotrophins. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR is not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1. SUBCELLULAR LOCATION: Membrane; single-pass type I membrane protein. DOMAIN: Death domain is responsible for interaction with RANBP9. PTM: N- and O-glycosylated. PTM: Phosphorylated on serine residues. SIMILARITY: Contains 1 death domain. SIMILARITY: Contains 4 TNFR-Cys repeats.

Long Name

Nerve Growth Factor Receptor

Alternate Names

CD271, NGF R, p75 NTR, TNFRSF16

Gene Symbol

NGFR

Additional NGFR/TNFRSF16 Products

Product Documents for NGFR/TNFRSF16/p75NTR Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NGFR/TNFRSF16/p75NTR Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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