Olig2 Antibody (HL1072)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13712

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
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NBP3-13712
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1072

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Olig2 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Olig2. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Olig2 Antibody (HL1072)

Immunocytochemistry/Immunofluorescence: Olig2 Antibody (HL1072) [NBP3-13712] - Olig2 antibody detects Olig2 protein at oligodendrocyte by immunofluorescent analysis. Sample: DIV10 rat cortical neuron-no AraC cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Olig2 stained by Olig2 antibody (NBP3-13712) diluted at 1:500. Red: Tau, a marker, stained by Tau antibody [GT287] diluted at 1:500.
Immunohistochemistry-Paraffin: Olig2 Antibody (HL1072) [NBP3-13712] - Olig2 antibody [HL1072] detects Olig2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Olig2 stained by Olig2 antibody [HL1072] (NBP3-13712) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Frozen: Olig2 Antibody (HL1072) [NBP3-13712] - Olig2 antibody [HL1072] detects Olig2 protein at nucleus by immunohistochemical analysis. Sample: Frozen-sectioned mouse brain. Green: Olig2 stained by Olig2 antibody [HL1072] (NBP3-13712) diluted at 1:100. Blue: Fluoroshield with DAPI. Antigen Retrieval: ice-cold MeOH for 5 min
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Applications for Olig2 Antibody (HL1072)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000
Application Notes
IHC-Fr Assay dependent

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Olig2

Olig2 is a transcription factor which is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. OIlig2 is expressed in the ventral spinal cord as early as 9.5 dpc and is scattered in the mantle zone, likely corresponding to oligodendrocyte progenitors migrating out from their site of origin. (Arnett et al., 2004) From 10.5 through 14.5 dpc, Olig2 is expressed in numerous cells in the ventricular and subventricular zones of the lateral and medial ganglionic eminences, suggesting that expression might not be limited to the oligodendrocytic lineage. Olig2 has further been shown to be critical in the proliferation of malignant glioma in brain tumors (Ligon et al., 2007).

Long Name

Oligodendrocyte Lineage Transcription Factor 2

Alternate Names

BHLHB1, PRKCBP2, RACK17

Gene Symbol

OLIG2

Additional Olig2 Products

Product Documents for Olig2 Antibody (HL1072)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Olig2 Antibody (HL1072)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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