P2Y12/P2RY12 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33870
Key Product Details
Validated by
Knockout/Knockdown, Biological Validation
Species Reactivity
Validated:
Human, Canine, Feline
Cited:
Human, Mouse, Primate - Macaca mulatta (Rhesus Macaque)
Applications
Validated:
Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for P2Y12/P2RY12 Antibody
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: KSFRNSLISMLKCPNSATSLSQDNRKKEQDGGDPNEETPM
Reactivity Notes
Feline, Canine reactivity reported from verified customer reviews.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for P2Y12/P2RY12 Antibody
Western Blot: P2Y12/P2RY12 Antibody [NBP2-33870] - Quantitative biochemical measurements of P2Y12/P2RY12 protein and mRNA in human brains. Western blot measurements of P2Y12/P2RY12 levels in MTG samples from LP, HP and AD brains. Representative western blot image of P2Y12/P2RY12 polypeptide of MTG protein extracts identified with Novus antibody. Blots were normalized for levels of beta actin. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/1422-0067/21/2/678), licensed under a CC-BY license.
Immunocytochemistry/Immunofluorescence: P2Y12/P2RY12 Antibody [NBP2-33870] - Green-P2Y12/P2RY12, Blue-DAPI. 1:200 dilution for fixed tissue sections of feline optical nerve. ICC/IF image submitted by a verified customer review.
Applications for P2Y12/P2RY12 Antibody
Application
Recommended Usage
Immunocytochemistry/Immunofluorescence
-validated from a verified customer review
Immunohistochemistry
1:1000 - 1:2500
Immunohistochemistry-Frozen
-validated from a verified customer review
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Western Blot
- reported in scientific literature (PMID:31968618).
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 3.3 using NBP2-33870 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: P2Y12/P2RY12
Long Name
P2Y Purinergic Receptor 12
Alternate Names
ADPG-R, HORK3, P2RY12, SP1999
Gene Symbol
P2RY12
Additional P2Y12/P2RY12 Products
Product Documents for P2Y12/P2RY12 Antibody
Product Specific Notices for P2Y12/P2RY12 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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