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p70 S6 Kinase/S6K Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23649

Novus Biologicals, part of Bio-Techne
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NBP2-23649SS
NBP2-23649

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Rabbit

Applications

Validated:

ICC/IF (Negative), Peptide ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Summary for p70 S6 Kinase/S6K Antibody

Immunogen

Synthetic peptide made to a C-terminal sequence of the human p70 S6 Kinase protein (between amino acids 350-472). [UniProt P23443-2].

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: canine (87%), equine (81%).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for p70 S6 Kinase/S6K Antibody

Western Blot: p70 S6 Kinase/S6K Antibody [NBP2-23649] - Western blot Image of anti-p70 S6 Kinase. Whole cell protein from HeLa (lane 1), HEK293 (lane 2) and MCF7 (lane 3) was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-p70 S6 Kinase in 1% milk, and detected with an anti-goat HRP secondary antibody using chemiluminescence.

Applications for p70 S6 Kinase/S6K Antibody

Application
Recommended Usage

Peptide ELISA

1:100 - 1:1000

Western Blot

1:100 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Whole sera with 50% Glycerol

Preservative

0.025% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: p70 S6 Kinase

S6K encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized.

Alternate Names

p70-alpha, RPS6KB1, S6K1, STK14A

Gene Symbol

RPS6KB1

Additional p70 S6 Kinase Products

Product Documents for p70 S6 Kinase/S6K Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for p70 S6 Kinase/S6K Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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