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Key Product Details

Species Reactivity

Validated:

Epitope Tag

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Negative), Western Blot

Label

HRP

Antibody Source

Polyclonal Goat IgG

Concentration

1.0 mg/ml

Product Summary for S-Tag Epitope Tag Antibody [HRP]

Immunogen

Goats were immunized with S-Tag (KETAAAKFERQHMDS) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for S-Tag Epitope Tag Antibody [HRP]

Western Blot: S-Tag Epitope Tag Antibody [HRP] [NB600-510] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).

Applications for S-Tag Epitope Tag Antibody [HRP]

Application
Recommended Usage

ELISA

1:10000 -1:100000

Immunocytochemistry/ Immunofluorescence

1:200-1:500

Western Blot

1:1000-1:30000
Application Notes
Immunohistochemistry Not applicable
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 0.2% BSA

Preservative

0.05% Pro-Clean 400

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: S-Tag Epitope Tag

The S-Tag epitope tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized S-Tag epitope represents the amino acid sequence KETAAAKFERQHMDS derived from pancreatic ribonuclease A.

Alternate Names

KETAAAKFERQHMDS, S 7 Epitope tag

Additional S-Tag Epitope Tag Products

Product Documents for S-Tag Epitope Tag Antibody [HRP]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for S-Tag Epitope Tag Antibody [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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