Key Product Details

Species Reactivity

Validated:

SARS-CoV, SARS-CoV-2

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 001

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SARS Nucleocapsid Protein Antibodies »

Product Summary for SARS Nucleocapsid Protein Antibody (001)

Immunogen

Recombinant SARS-CoV Nucleoprotein/NP Protein

Reactivity Notes

This antibody has cross-reactivity in ELISA and WB with:
SARS-CoV-2 Nucleocapsid Protein

No cross-reactivity in ELISA with:
MERS-CoV Nucleoprotein protein
HCoV-229E Nucleoprotein protein
HCoV-NL63 Nucleoprotein protein
HCoV-HKU1(isolate N5) Nucleoprotein protein
HCoV-OC43 Nucleoprotein

Specificity

Has cross-reactivity in ELISA and WB with:SARS-CoV-2 Nucleocapsid ProteinHas cross-reactivity in ELISA with:SARS-CoV-2 Omicron (B.1.1.529) Nucleocapsid ProteinSARS-CoV-2 Nucleocapsid ProteinSARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(R203K, G204R) ProteinSARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(I292T) ProteinSARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(D3L, R203K, G204R, S235F) ProteinSARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(D3L, S235F) ProteinSARS-CoV-2 Alpha(B.1.1.7/ B.1.237) Nucleocapsid(S194L) ProteinSARS-CoV-2 Alpha/beta(B.1.1.7/ B.1.351/ A.2.2) Nucleocapsid(P13L) ProteinSARS-CoV-2 Beta(B.1.351/B.1.351.2/b.1.351.3/B.1.427/B.1.429) Nucleocapsid (T205I) ProteinSARS-CoV-2 Gamma(P.1/ P.1.1/ P.1.2) Nucleocapsid (P80R) ProteinSARS-CoV-2 Eta(B.1.525) Nucleocapsid (A12G, T205I) ProteinSARS-CoV-2 (B.1.617) Nucleocapsid (D377Y) ProteinSARS-CoV-2 (B.1.617) Nucleocapsid (R203M, D377Y) ProteinSARS-CoV-2 Delta(B.1.617.2) Nucleocapsid (D63G, R203M, D377Y) ProteinSARS-CoV-2 (B.1.617.3) Nucleocapsid (P67S, R203M, D377Y) ProteinNo cross-reactivity in ELISA with:MERS-CoV Nucleocapsid proteinHCoV-229E Nucleocapsid proteinHCoV-NL63 Nucleocapsid proteinHCoV-HKU1(isolate N5) Nucleocapsid proteinHCoV-OC43 Nucleocapsid protein

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for SARS Nucleocapsid Protein Antibody (001)

Western Blot: SARS-CoV-2 Nucleocapsid Antibody (001) [NBP2-90988] - 1:2000 dilution. Lane A: SARS-CoV-2 NP Protein (30ng). Lane B: SARS-CoV NP Protein (5ng). Lane C: SARS-CoV-2 (2019-nCoV) NP Protein (30ng). Lane D: SARS-CoV-2 (2019-nCoV) NP Protein (5ng). Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions.
Immunocytochemistry/Immunofluorescence: SARS Nucleocapsid Protein Antibody (001) [NBP2-90988] - Nucleocapsid overexpressed HEK293 Cells were stained with purified anti-SARSNucleocapsid Rabbit Mab,then a HRPconjugated second step antibody
Immunocytochemistry/Immunofluorescence: SARS Nucleocapsid Protein Antibody (001) [NBP2-90988] - Analysis of SARS-COV-2 Nucleocapsid overexpressed HEK293 Cells were stained with purified anti-SARS Nucleocapsid Rabbit Mab,then a Alexa Fluor®488-conjugated second step antibody.
View 1 more images

Applications for SARS Nucleocapsid Protein Antibody (001)

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Immunohistochemistry-Paraffin

1:5000-1:20000

Western Blot

1:5000-1:20000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SARS Nucleocapsid Protein

It has recently been shown that SARS is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics.

Alternate Names

N, N protein, N structural protein, NC, Nucleocapsid protein, Nucleoprotein, Protein N, SARS coronavirus N protein, SARS coronavirus nucleocapsid protein, SARS CoV, SARS CoV N protein, SARS CoV nucleocapsid protein, SARS N protein, SARS Nucleoprotein, SARSCoV, SARSCoV N protein, SARSCoV nucleocapsid protein, Severe acute respiratory syndrome, SARSCOV2 N protein, SARS-COV-2 nucleocapsid protein, SARS-COV-2 Nucleoprotein

Gene Symbol

N

Additional SARS Nucleocapsid Protein Products

Product Documents for SARS Nucleocapsid Protein Antibody (001)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SARS Nucleocapsid Protein Antibody (001)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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