Key Product Details

Species Reactivity

Validated:

SARS-CoV

Applications

ELISA, Flow Cytometry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 05

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SARS Nucleocapsid Protein Antibodies »

Product Summary for SARS Nucleocapsid Protein Antibody (05)

Immunogen

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant SARS Nucleocapsid Protein (Accession#: NP_828858.1; Met1-Ala422).

Specificity

Has cross-reactivity in ELISA and WB with:SARS-CoV-2 Nucleocapsid ProteinHas cross-reactivity in ELISA with:SARS-CoV-2 Omicron (B.1.1.529) Nucleocapsid ProteinSARS-CoV-2 Nucleocapsid ProteinSARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(R203K, G204R) ProteinSARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(I292T) ProteinSARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(D3L, R203K, G204R, S235F) ProteinSARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(D3L, S235F) ProteinSARS-CoV-2 Alpha(B.1.1.7/ B.1.237) Nucleocapsid(S194L) ProteinSARS-CoV-2 Alpha/beta(B.1.1.7/ B.1.351/ A.2.2) Nucleocapsid(P13L) ProteinSARS-CoV-2 Beta(B.1.351/B.1.351.2/b.1.351.3/B.1.427/B.1.429) Nucleocapsid (T205I) ProteinSARS-CoV-2 Gamma(P.1/ P.1.1/ P.1.2) Nucleocapsid (P80R) ProteinSARS-CoV-2 Eta(B.1.525) Nucleocapsid (A12G, T205I) ProteinSARS-CoV-2 (B.1.617) Nucleocapsid (D377Y) ProteinSARS-CoV-2 (B.1.617) Nucleocapsid (R203M, D377Y) ProteinSARS-CoV-2 Delta(B.1.617.2) Nucleocapsid (D63G, R203M, D377Y) ProteinSARS-CoV-2 (B.1.617.3) Nucleocapsid (P67S, R203M, D377Y) ProteinNo cross-reactivity in ELISA with:MERS-CoV Nucleocapsid proteinHCoV-229E Nucleocapsid proteinHCoV-NL63 Nucleocapsid proteinHCoV-HKU1(isolate N5) Nucleocapsid proteinHCoV-OC43 Nucleocapsid protein

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for SARS Nucleocapsid Protein Antibody (05)

Western Blot: SARS Nucleocapsid Protein Antibody (05) [NBP3-06413] - Anti-SARS-NP mouse monoclonal antibody at 1:1000 dilution.Lane A: SARS-CoV NP Protein (30ng)Lane B: SARS-CoV NP Protein (5ng)Lane C: SARS-CoV-2 (2019-nCoV) NP Protein (30ng)Lane D: SARS-CoV-2 (2019-nCoV) NP Protein (5ng)Secondary: Goat Anti-Mouse IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.

Applications for SARS Nucleocapsid Protein Antibody (05)

Application
Recommended Usage

ELISA

1:1000-1:2000

Flow Cytometry

1:25-1:100

Immunohistochemistry-Paraffin

1:1000-1:5000

Western Blot

1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SARS Nucleocapsid Protein

It has recently been shown that SARS is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139 kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics.

Alternate Names

N, N protein, N structural protein, NC, Nucleocapsid protein, Nucleoprotein, Protein N, SARS coronavirus N protein, SARS coronavirus nucleocapsid protein, SARS CoV, SARS CoV N protein, SARS CoV nucleocapsid protein, SARS N protein, SARS Nucleoprotein, SARSCoV, SARSCoV N protein, SARSCoV nucleocapsid protein, Severe acute respiratory syndrome

Gene Symbol

N

Additional SARS Nucleocapsid Protein Products

Product Documents for SARS Nucleocapsid Protein Antibody (05)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SARS Nucleocapsid Protein Antibody (05)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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