SARS Nucleocapsid Protein Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44205
Key Product Details
Species Reactivity
Validated:
SARS-CoV
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
LYOPH mg/ml
Product Summary for SARS Nucleocapsid Protein Antibody
Immunogen
This SARS Nucleocapsid Protein Antibody was developed by immunizing rabbits with a full-length recombinant protein from the N (SARS Nucleocapsid) for the Human SARS coronavirus (Uniprot#: P59595)
Reactivity Notes
SARS Coronavirus. Immunogen displays the following percentage of sequence identity for non-tested species: COVID-19 (91%)
Specificity
The product was purified from monospecific antiserum by protein A affinity purification.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
The recombinant nucleocapsid protein was prepared as follows: SUMO-Nucleocapsid fusion was expressed in E.coli in LB medium and purified using Ni-NTA resin (Qiagen) affinity chromatography. After the fusion was cleaved by the SUMO Protease, the SUMO tag and protease were subtracted from the nucleocapsid using MAC and the nucleocapsid was finally purified using Cation Exchange Chromatography with the Macro-Prep High S resin (BioRad) and size exclusion chromatography.
NBP2-44205 is manufactured exclusively by Rockland Immunochemicals.
This protein A purified antibody is directed against SARS Coronavirus Nucleocapsid (N) protein. The product was purified from monospecific antiserum by protein A affinity purification
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
NBP2-44205 is manufactured exclusively by Rockland Immunochemicals.
This protein A purified antibody is directed against SARS Coronavirus Nucleocapsid (N) protein. The product was purified from monospecific antiserum by protein A affinity purification
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for SARS Nucleocapsid Protein Antibody
Western Blot: SARS Nucleocapsid Protein Antibody [NBP2-44205] - Western blot using Protein A Purified anti-SARS CoV Nucleocapsid (N) protein antibody shows detection of a 46 kDa band corresponding to the protein. Approx. 100 ng of protein was loaded for SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:5,000 dilution of the antibody at room temperature for 1 h followed by detection using IRDy(TM) 800 labeled Goat-a-Rabbit IgG [H&L] (611-132-122) diluted 1:10,000. The fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Immunocytochemistry/Immunofluorescence: SARS Nucleocapsid Protein Antibody [NBP2-44205] - Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody, showing viral protein synthesis. VeroE6 cells were infected with rSARS-CoV-2. Mock: non-infected cells. At 48 h.p.i, cells were fixed and prepared for immunofluorescence staining with primary antibodies directed against double-stranded RNA (dsRNA) [green] and SARS-CoV Nucleocapsid (N) [red]. Nuclear Counterstain DAPI [blue].
Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NBP2-44205] - Immunohistochemistry results showing positive staining in placenta tissue using Anti-SARS-CoV Nucleocapsid (N) Antibody.
Applications for SARS Nucleocapsid Protein Antibody
Application
Recommended Usage
ELISA
1:10000 - 1:50000
Immunocytochemistry/ Immunofluorescence
1:10 - 1:500
Immunohistochemistry
1:100 - 1:6000
Western Blot
1:2000 - 1:10000
Application Notes
Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 46 kDa in size corresponding to SARS Nucleocapsid (N) protein by western blotting in the appropriate cell lysate or extract. ELISA and lateral flow format has been used to detect virus in extracts from nasal and throat swabs and saliva. IF has been used to determine the presence or absence of virus entering cells especially when This antibody has the ability to neutralize the virus and thereby protect cells from the uptake of live virus. Others have demonstrated the utility of the antibody in flow cytometry studies.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
For the 500 ug size, we recommend reconstituting with 100 ul deionized water. The 0.1 mg size is provided in liquid format (0.02 ml).
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: SARS Nucleocapsid Protein
Alternate Names
N, N protein, N structural protein, NC, Nucleocapsid protein, Nucleoprotein, Protein N, SARS coronavirus N protein, SARS coronavirus nucleocapsid protein, SARS CoV, SARS CoV N protein, SARS CoV nucleocapsid protein, SARS N protein, SARS Nucleoprotein, SARSCoV, SARSCoV N protein, SARSCoV nucleocapsid protein, Severe acute respiratory syndrome
Gene Symbol
N
Additional SARS Nucleocapsid Protein Products
Product Documents for SARS Nucleocapsid Protein Antibody
Product Specific Notices for SARS Nucleocapsid Protein Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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