Key Product Details

Species Reactivity

Validated:

SARS-CoV, SARS-CoV-2

Cited:

Human, Virus - SARS-CoV-2

Applications

Validated:

Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Simple Western, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all SARS Spike Protein Antibodies »

Product Specifications

Immunogen

The antibody was developed by immunizing rabbits with a synthetic peptide corresponding to amino acids 1124-1140 (C-GNCDVVIGIVNNTVYDP) from the S (Spike glycoprotein)(Spike protein S2') for the Human SARS coronavirus (Genbank accession no. YP_009724390)

Specificity

Full length Spike protein transfected into UM92 cells was used as a positive control and an approximate 139 kDa band was observed. Dot Blot results using recombinant proteins for cross-reactivity testing revealed high reactivity to SARS-CoV-2 Spike Protein, 1000-1200 a.a. (NBP2-90973) and low/no reactivity towards MERS Spike 2 or H1N1 (NBP1-99041).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SARS Spike Protein Antibody

Simple Western: Sars Spike Protein Antibody [NB100-56578] - Simple Western lane view shows recombinant SARS-CoV-2 Spike S2 Protein, loaded at 50 ng/mL. A specific band was detected for SARS-CoV-2 Spike S2 Protein at approximately 58 kDa (as indicated) using a serial dilution of Rabbit Anti-SARS-CoV Spike Protein Polyclonal Antibody (Catalog # NB100-56578) followed by incubation with HRP-conjugated Anti-Rabbit IgG Secondary Antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Western Blot: Sars Spike Protein Antibody [NB100-56578] - SARS-CoV-2 spike overexpressed 293T cell lysate. Antibody dilution: 1:3000. Two bands for full length spike and cleaved S2 are shown, respectively. Western blot image submitted by a verified customer review.
Western Blot: SARS Spike Protein Antibody [NB100-56578] - Western blot shows SARS-CoV-1 Spike (Catalog # 10581-CV), SARS CoV-2 Spike (Catalog # 10586-CV), SArS-CoV-2 Spike S2 Subunit (Catalog # 10584-CV). Membrane was probed with 0.5 ug/mL of SARS Spike Protein Antibody (Catalog # NB100-56578) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for SARS-CoV-2 Spike S2 Subunit at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. Internally validated Western blot.
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Applications for SARS Spike Protein Antibody

Application
Recommended Usage

Immunocytochemistry/Immunofluorescence

reported in scientific literature (PMID 16081529)

Immunohistochemistry

reported in scientific literature (PMID 34357881)

Simple Western

1:40 - 1:160

Western Blot

0.05 - 1.0 ug/mL

Reviewed Applications

Read 1 review rated 5 using NB100-56578 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SARS Spike Protein

It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The glycosilated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients.

Alternate Names

E2, E2 glycoprotein, Human coronavirus spike glycoprotein, Peplomer protein, S, S glycoprotein, SARS coronavirus S protein, SARS coronavirus Spike Protein, SARS CoV, SARS CoV S glycoprotein, SARS CoV Spike protein, SARSCoV, SARS-CoV S protein, SARS-COV Spike, Severe acute respiratory syndrome spike glycoprotein, Severe acute respiratory syndrome virus spike glycoprotein, Spike glycoprotein, VGL2, COVID-19 Spike, SARS-COV-2 S protein, SARS-COV-2 Spike glycoprotein, SARSCOV2 Spike protein

Additional SARS Spike Protein Products

Product Documents for SARS Spike Protein Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SARS Spike Protein Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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