Siglec-2/CD22 Antibody (2H1C4) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37327
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 2H1C4
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Purified recombinant fragment of human Siglec-2/CD22 (AA: 621-725) expressed in E. Coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
95.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Siglec-2/CD22 Antibody (2H1C4) - BSA Free
Western Blot: Siglec-2/CD22 Antibody (2H1C4) [NBP2-37327] - Analysis using CD22 mouse mAb against L1210 (1), Hela (2), HEK293 (3), Jurkat (4), OCM-1 (5), A432 (6) and NIH/3T3 (7) cell lysate.
Immunocytochemistry/Immunofluorescence: Siglec-2/CD22 Antibody (2H1C4) [NBP2-37327] - Immunofluorescence analysis of Hela cells using CD22 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (2H1C4) [NBP2-37327] - Analysis of cerebellum tissues using CD22 mouse mAb with DAB staining.
Applications for Siglec-2/CD22 Antibody (2H1C4) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Flow Cytometry
1:200 - 1:400
Immunocytochemistry/ Immunofluorescence
1:200 - 1:1000
Immunohistochemistry
1:200 - 1:1000
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Siglec-2/CD22
Long Name
Sialic Acid Binding Ig-like Lectin 2
Alternate Names
BL-CAM, CD22, Siglec2
Gene Symbol
CD22
Additional Siglec-2/CD22 Products
Product Documents for Siglec-2/CD22 Antibody (2H1C4) - BSA Free
Product Specific Notices for Siglec-2/CD22 Antibody (2H1C4) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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