Siglec-2/CD22 Antibody (RFB4) [PE]

Validated:

Cited:

Validated:

Cited:

PE (Excitation = 488 nm, Emission = 575 nm)

Monoclonal Mouse IgG₁ Clone # RFB4

Please see the vial label for concentration. If unlisted please contact technical services.

135 kDa type I integral membrane protein

Use in Canine reported in scientific literature (PMID:31826004).

This antibody recognizes surface Siglec-2/CD22 expressed by mature peripheral B-cells and cytoplasmatic Siglec-2/CD22 expressed by precursor B-cells. The sensitivity is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity).For this purpose, a mAb-concentration range is selected to include both the saturation point (i.e. the mAb dilution expected to bind all epitopes on the target cell) and the detection threshold (i.e. the mAb dilution expected to represent the least amount of mAb needed to detect an identical percentage of cells). In practice, 50 ul of leukocytes containing 10^7 cells/ml are stained with 20 ul mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold. The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold. In addition and to control lot-to-lot variation, the given lot is compared and adjusted to fluorescence standards with defined intensity.

Monoclonal

Mouse

IgG₁

For stability reasons this monoclonal antibody solution contains sodium azide. These reagents should be stored at 2-8C (DO NOT FREEZE!) and protected from prolonged exposure to light. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product. Stability of the reagent: Please refer to the expiry date printed onto the vial. The use of the reagent after the expiration date is not recommended.

Protein A or G purified

PBS (pH 7.2) and 1.0% (w/v) BSA

0.05% Sodium Azide

Please see the vial label for concentration. If unlisted please contact technical services.

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at 4C in the dark.