TOMM20 Antibody (10G6I8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15750
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 10G6I8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for TOMM20 Antibody (10G6I8)
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 20-145 of human TOMM20 (NP_055580.1). YCIYFDRKRRSDPNFKNRLRERRKKQKLAKERAGLSKLPDLKDAEAVQKFFLEEIQLGEELLAQGEYEKGVDHLTNAIAVCGQPQQLLQVLQQTLPPPVFQMLLTKLPTISQRIVSAQSLAEDDVE
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TOMM20 Antibody (10G6I8)
Western Blot: TOMM20 Antibody (6E0N2) [NBP3-15750] - Western blot analysis of extracts of various cell lines, using TOMM20 antibody (NBP3-15750) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/Immunofluorescence: TOMM20 Antibody (6E0N2) [NBP3-15750] - Confocal immunofluorescence analysis of HeLa cells using TOMM20 Rabbit mAb (NBP3-15750) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: TOMM20 Antibody (6E0N2) [NBP3-15750] - Immunohistochemistry of paraffin-embedded human colon carcinoma using TOMM20 antibody (NBP3-15750) at dilution of 1:400 - 3200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Applications for TOMM20 Antibody (10G6I8)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100 - 1:500
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Immunoprecipitation
-0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Western Blot
1:2000 - 1:6000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: TOMM20
Alternate Names
KIAA0016Outer mitochondrial membrane receptor Tom20, MAS20, MGC117367, Mitochondrial 20 kDa outer membrane protein, mitochondrial import receptor subunit TOM20 homolog, MOM19, TOM20, translocase of outer mitochondrial membrane 20 homolog (yeast), translocase of outer mitochondrial membrane 20 homolog type II
Gene Symbol
TOMM20
Additional TOMM20 Products
Product Documents for TOMM20 Antibody (10G6I8)
Product Specific Notices for TOMM20 Antibody (10G6I8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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