TOMM20 Antibody (ST04-72)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67501
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
ICC/IF, IHC-Fr
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # ST04-72
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within Human TOMM20 aa 1-145 / 145. (SwissProt: Q15388 Human; SwissProt: Q9DCC8 Mouse; SwissProt: Q62760 Rat)
Localization
Mitochondrion outer membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for TOMM20 Antibody (ST04-72)
Western Blot: TOMM20 Antibody (ST04-72) [NBP2-67501] - Analysis of TOMM20 on different lysates using anti-TOMM20 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: F9 Lane 4: PC12
Immunocytochemistry/Immunofluorescence: TOMM20 Antibody (ST04-72) [NBP2-67501] - Staining TOMM20 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: TOMM20 Antibody (ST04-72) [NBP2-67501] - Analysis of paraffin-embedded human liver tissue with Rabbit anti-TOMM20 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Applications for TOMM20 Antibody (ST04-72)
Application
Recommended Usage
Flow Cytometry
1:50-1:100
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry-Paraffin
1:50-1:800
Western Blot
1:500-1:2000
Reviewed Applications
Read 1 review rated 5 using NBP2-67501 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TOMM20
Alternate Names
KIAA0016Outer mitochondrial membrane receptor Tom20, MAS20, MGC117367, Mitochondrial 20 kDa outer membrane protein, mitochondrial import receptor subunit TOM20 homolog, MOM19, TOM20, translocase of outer mitochondrial membrane 20 homolog (yeast), translocase of outer mitochondrial membrane 20 homolog type II
Gene Symbol
TOMM20
Additional TOMM20 Products
Product Documents for TOMM20 Antibody (ST04-72)
Product Specific Notices for TOMM20 Antibody (ST04-72)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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