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V5 Epitope Tag Alexa Fluor® 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB8926G

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Multi-Species

Applications

Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Rabbit IgG Clone # 1036H

Product Summary for V5 Epitope Tag Alexa Fluor® 488-conjugated Antibody

Immunogen

V5 epitope tag peptide
Accession # Y138513

Specificity

Detects N-terminal and C-terminal V5 epitope-tagged proteins in Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for V5 Epitope Tag Alexa Fluor® 488-conjugated Antibody

Detection of V5 Epitope Tag antibody in HEK293 Human Cell Line Transfected with V5-tagged Proteins antibody by Flow Cytometry.

Detection of V5 Epitope Tag in HEK293 Human Cell Line Transfected with V5-tagged Proteins by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with V5-tagged proteins was stained with Rabbit Anti-V5 Epitope Tag Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB8926G, filled histogram) or isotype control antibody (Catalog # IC1051G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for V5 Epitope Tag Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with V5-tagged proteins
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: V5 Epitope Tag

The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

Alternate Names

GKPIPNPLLGLDST Epitope Tag, Parainfluenza virus 5 V/P, Paramyxovirus SV5

Entrez Gene IDs

3160800 (Viral)

Gene Symbol

V/P

UniProt

Additional V5 Epitope Tag Products

Product Documents for V5 Epitope Tag Alexa Fluor® 488-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for V5 Epitope Tag Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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