Skip to main content

Key Product Details

Species Reactivity

Epitope Tag

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Radioimmunoassay, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # SV5-P-K

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Pk (V5) Epitope Tag (GKPIPNPLLGLDST).

Reactivity Notes

Paramyxovirus Simian Virus 5

Specificity

Recognises a 14 amino acid sequence (GKPIPNPLLGLDST) derived from the P and V proteins of the paramyxoVirus simian virus 5.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for V5 Epitope Tag Antibody (SV5-P-K) - Chimeric - Azide and BSA Free

ELISA: V5 Epitope Tag Antibody (SV5-P-K) [NBP2-52653] - ELISA of anti-epitope tag antibodies on multi-tag protein Plate coated with 5ug/ml of Multitag protein. Primary antibody loaded at 1000 ng/ml in first well and an 11 point serial dilution performed in duplicate for each antibody with last well left blank. Signal detection was performed using HRP-conjugated goat anti-mouse.

Applications for V5 Epitope Tag Antibody (SV5-P-K) - Chimeric - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunohistochemistry-Frozen

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
This is a chimeric rabbit antibody, based on the original mouse IgG1 format, created for improved compatibility with existing reagents, assays and techniques.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: V5 Epitope Tag

The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

Alternate Names

GKPIPNPLLGLDST Epitope Tag, Parainfluenza virus 5 V/P, Paramyxovirus SV5

Additional V5 Epitope Tag Products

Product Documents for V5 Epitope Tag Antibody (SV5-P-K) - Chimeric - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for V5 Epitope Tag Antibody (SV5-P-K) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...